XBridge Protein BEH SEC, 125 Å Columns, containing 3.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 1,000 – 80,000 Daltons. Note: P/N 1763591 contains an XBridge Protein BEH SEC, 125 Å, 3.5um 7.8 x 30 Guard Column with one vial the BEH125 SEC Protein Std (P/N 186006519)
Chemistry |
SEC |
Separation Mode |
SEC |
Particle Substrate |
Hybrid |
Bonding Technology |
SEC |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
3.5 µm |
Pore Size |
125 Å |
QC Tested |
Protein |
Format |
Guard Column |
System |
HPLC |
Particle Technology |
BEH |
Inner Diameter |
7.8 mm |
Length |
30 mm |
UNSPSC |
41115709 |
Brand |
XBridge |
Product Type |
Guards |
Units per Package |
1 pk |
XBridge Protein BEH SEC Guard Column, 125Å, 3.5 µm, 7.8 mm X 30 mm
Utilize the XBridge Protein BEH SEC Guard Column to extend column longevity. A piece of safety lab equipment called a guard column is positioned between the injector and the analytical column. It works by preventing suspended particles and chemical contaminants from entering the analytical column.
The same high-performance packing materials utilized in the production of all Waters analytical columns are also employed in the XBridge Protein BEH SEC Guard Column. It was created and constructed in particular for circumstances when a greater capacity for biological fluid cleansing and/or temperature control is required.
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The XBridge Protein BEH SEC Guard Column, like other Waters products, is made in a cGMP, ISO 9001-certified facility using ultra-pure chemicals. Additionally, the packing material for the XBridge Protein BEH SEC Guard Column is put through chromatographic testing using neutral, acidic, and basic analytes for each batch. The results are once again held to the highest standards and the tightest specification ranges to ensure consistently excellent, repeatable performance.
You may also want to invest in the TruView LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk. TruView LCMS Certified Glass Vials use a production procedure that minimizes adsorption. High recoveries of polar analytes are produced by this technique because the circumstances limit the concentration of free ions on the glass surface. Using a number of methods, including LC/UV and UPLC/MS, all vials are extensively evaluated for dimensional features, chemical purity, and adsorption. In this test, MS detection is used in an objective manner to check for any ionized masses independent of their source. The total ion count and the existence of clusters in the high mass range are the two criteria defined for tests that are done in scan mode.
What Causes Adsorption And Why?
Because molecules on the surface are subject to more inward force than those in the internal core, they are more likely to stick together when in touch.