SKU: 186007636
XBridge Protein  BEH  SEC  Column, 125Å, 3.5 µm, 7.8 mm X 150 mm

XBridge Protein BEH SEC Column | 186007636

Streamline Purchasing with Customized Quotes

Streamline Purchasing with Customized Quotes

Shop on Waters.com and add items to your cart

Navigate to the Shopping Cart

Select Request a Quote to get your quote instantly

Learn More

Product Description

XBridge Protein BEH SEC, 125 Å Columns, containing 3.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns.   They are best suited to separate proteins ranging from 1,000 – 80,000 Daltons.  Note: P/N 176003592 contains an XBridge Protein BEH SEC, 125 Å, 3.5um  7.8 x 150  Column with one vial the BEH125 SEC Protein Std (P/N 186006519)

Specifications

  • Chemistry

    SEC

  • Separation Mode

    SEC

  • Particle Substrate

    Hybrid

  • Bonding Technology

    SEC

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    3.5 µm

  • Endfitting Type

    Waters

  • Pore Size

    125 Å

  • QC Tested

    Protein

  • Format

    Column

  • System

    HPLC

  • Particle Technology

    BEH

  • Inner Diameter

    7.8 mm

  • Length

    150 mm

  • UNSPSC

    41115709

  • Brand

    XBridge

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

What do you want to do?

What do you want to do?
Need Help?

Related Products

Back To Top Back To Top

XBridge Protein BEH SEC Column, 125Å, 3.5 µm, 7.8 mm X 150 mm

The Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating used in our successful series of HPLC-based SEC columns are employed in the XBridge Protein BEH SEC, 125 Columns, featuring 3.5um particles. They're best for separating proteins with a Dalton range of 1,000 to 80,000. Unlike silica-based SEC Columns, the XBridge Protein BEH SEC Column has less non-desired protein and column interactions, allowing the column to have a longer column life.

The XBridge Protein BEH SEC Column's diol-bonded covering provides greater flow and pressure tolerance for increased sample throughput on HPLC systems. The combination of BEH base particle technology and diol bonding methods allows for better column stability, performance, and lifetime.

The XBridge Protein BEH SEC Column allows for a smooth transition to UltraPerformance LC Technology, and this equipment is built to give maximum productivity. The XBridge Protein BEH SEC Column, like all Waters equipment, is dependable and reproducible because it is manufactured in cGMP, ISO9001, and ISO13485 certified facilities. Every piece of equipment goes through thorough testing at every level and phase of production to ensure the best possible quality. The column is an important piece of laboratory equipment because it has unrivaled column longevity and the ability to function with a wide variety of mobile phase pH and temperatures.

Shop for lab equipment, and find our complete range of XBridge columns across a wide range of applications through the catalog on our website. Please feel free to reach out to a member of our global team, and we would be happy to assist you with any queries.

Related to the XBridge Protein BEH SEC Column, you might want to review XBridge Protein BEH SEC Guard Column, 125Å, 3.5 µm, 7.8 mm X 30 mm; XBridge Protein BEH SEC, 125 Å Columns, containing 3.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns.

What Is SEC And How Does It Work?

Size exclusion chromatography (SEC) is a Liquid Chromatography technique that is often used to monitor protein aggregates during the commercialization phase of a biotherapeutic protein. Monoclonal antibodies are an example of this. The method, also known as molecular sieve chromatography, divides molecules into groups depending on their size and, in certain situations, molecular weight. Large molecules or macromolecular complexes, such as proteins and industrial polymers, are commonly used. For the separation of proteins with varying hydrodynamic radii, HPLC-based SEC procedures typically use columns filled with >3 m particles of acceptable pore size. The proteins are best separated in solution based on their relative sizes, with bigger aggregation species eluting before the monomer medication of interest.