XBridge Protein BEH SEC Column, 200Å, 3.5 µm, 7.8 mm X 300 mm, 1/pk

Crafted to deliver superior performance to other silica-based size-exclusion chromatography (SEC) offerings, Waters’ XBridge Protein BEH SEC Column, 200Å, 3.5 µm, 7.8 mm X 300 mm allows chromatographers to easily develop and transfer SEC methods based on laboratory instrumentation, required protein component resolution, and sample throughput requirements. These columns were designed for use on HPLC instrumentation and to complement existing smaller particle-sized, UPLC-based SEC columns for SEC of proteins. For the discovery, development, and quality assessment of protein-based biotherapeutics, XBRidge Protein BEH SEC Columns support reliable, high resolving SEC methods.

With the ability to separate proteins from approximately 10k to 1500k Daltons, columns are flow and pressure tolerant for increased sample throughput on HPLC systems when compared with other traditional, silica-based SEC columns with particles >5 µm. This is all done while providing higher throughput capability to chromatographers.

The BEH particle technology used in this column is used in chromatography for a range of biological compounds with stability and performance attributes that are not found in many other traditional, 100% silica-based particles. By combining this BEH base particle with the innovative diol bonding process in these columns, you are able to achieve improved column stability, performance, and lifetime that is unprecedented in most SEC columns.

Compared to other silica-based SEC columns, fewer charged silanols exist on the diol coated BEH particles contained in XBridge Protein BEH SEC columns, which means less non-desirable ion interactions will occur between the protein and the SEC particle.

All Waters lab equipment, including any HPLC or UPLC SEC particles, are synthesized in ISO-certified manufacturing facilities using high-quality raw materials. They are thoroughly quality control tested throughout the synthetic process, and each material is tested against relevant proteins in order to ensure batch to batch consistency and offer confidence in validated methods.