Waters Glycoprotein BEH Amide 300A, 1.7μm columns were primarily designed for the analysis of intact glycoproteins, glycoprotein fragments, and glycopeptides. Each batch of Glycoprotein BEH Amide 300A 1.7μm material is specifically quality control tested with the Waters Glycoprotein Performance Test Standard (P/N 86008010 that includes a mixture of Ribonuclease A as well as Ribonulease B glycoforms) to help ensure batch to batch consistency.
Chemistry |
Amide |
Separation Mode |
Hydrophilic Interaction (HILIC) |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
10 pH |
Endcapped |
No |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
300 Å |
QC Tested |
Glycoprotein |
Format |
Column |
Surface Area |
185 |
System |
UPLC |
Particle Technology |
BEH |
USP Classification |
L68 |
Inner Diameter |
2.1 mm |
Length |
50 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Application |
Glycan |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC Glycoprotein BEH Amide Column, 300Å, 1.7 µm, 2.1 mm X 50 mm, 1/pk
The primary function of the Waters Glycoprotein BEH Amide 300A, 1.7 μm columns was to analyze whole glycoproteins, glycoprotein fragments, and glycopeptides. To maintain consistency from batch to batch, each batch of Glycoprotein BEH Amide 300A 1.7 μm material is carefully quality-controlled and tested using the Waters Glycoprotein Performance Test Standard (P/N 86008010, which contains a mixture of Ribonuclease A and Ribonulease B glycoforms).
Unprecedented levels of peak asymmetry, efficiency, and chemical stability are offered by ACQUITY UPLC BEH Columns. These strong columns can function in circumstances of severe pH and are available in both reversed-phase and HILIC, with chemistries that enable selectivity for numerous small-molecule molecules. The ACQUITY UPLC BEH Columns' capacity to withstand harsh pH conditions means that a wide pH range can be used to affect the retention, selectivity, and sensitivity of chemicals that can be ionized.
The cGMP, ISO 9002-compliant ACQUITY UPLC Glycoprotein BEH Amide Column is produced in a special Waters chromatography chemistry production facility under the most stringent circumstances possible. Chromatography components are created at the manufacturing facility, then loaded into columns and solid-phase extraction equipment to guarantee that you receive lab equipment of the highest caliber that produces trustworthy and consistent analytical findings.
Explore our website to find more Waters products and collections, as well as to shop for lab equipment based on your lab's requirements. You can also reach out to a member of our international support team through our website. Our customer service representatives are available to assist you if you have any questions or concerns.
You may also be interested in checking out GlycoWorks Reductive Amination High-throughput Sample Preparation Kit; The development and thorough testing of Waters' new GlycoWorks Glycan Sample Preparation Kits ensures that they will function in harmony with our current ACQUITY UPLC Glycan Application Solution to produce analyses of pre-column derivatized, labeled glycans that yield high-quality results. The use of Waters HILIC Solid Phase Extraction (SPE) material for the release of glycans from the protein and the subsequent separation of the labeled glycans from the free excess label is a crucial aspect of this product. This kit has been designed for high throughput applications and comes with a 96-well plate.
How Does Polarity Affect Chromatographic Separation?
When polar solvents are employed instead of non-polar solvents, polar molecules move farther because they are more strongly attracted to polar solvents. Comparisons and measurements can be conducted between the distance that spots move and the solvent's total movement.