SKU: 186007963
ACQUITY UPLC Glycoprotein BEH Amide Column, 300Å, 1.7 µm, 2.1 mm X 150 mm, 1/pk

ACQUITY UPLC Glycoprotein BEH Amide Column | 186007963


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Product Description

Waters Glycoprotein BEH Amide 300A, 1.7μm columns were primarily designed for the analysis of intact glycoproteins, glycoprotein fragments, and glycopeptides. Each batch of  Glycoprotein BEH Amide 300A 1.7μm material is specifically quality control tested with the Waters Glycoprotein Performance Test Standard (P/N 86008010 that includes a mixture of Ribonuclease A as well as Ribonulease B glycoforms) to help ensure batch to batch consistency.

Specifications

  • Chemistry

    Amide

  • Separation Mode

    Hydrophilic Interaction (HILIC)

  • Particle Substrate

    Hybrid

  • pH Range Min

    2 pH

  • pH Range Max

    10 pH

  • Endcapped

    No

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    300 Å

  • QC Tested

    Glycoprotein

  • Format

    Column

  • Surface Area

    90

  • System

    UPLC

  • Particle Technology

    BEH

  • USP Classification

    L68

  • Inner Diameter

    2.1 mm

  • Length

    150 mm

  • Carbon Load

    12 %

  • UNSPSC

    41115709

  • Application

    Glycan

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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ACQUITY UPLC Glycoprotein BEH Amide Column, 300Å, 1.7 µm, 2.1 mm X 150 mm, 1/pk

The Waters Glycoprotein BEH Amide 300A, 1.7m columns were created to analyze intact glycoproteins, glycoprotein fragments, and glycopeptides. To assure batch-to-batch consistency, each batch of Glycoprotein BEH Amide 300A 1.7m material is evaluated with the Waters Glycoprotein Performance Test Standard (P/N 86008010, which includes a mixture of Ribonuclease A and Ribonuclease B glycoforms). This is done to help ensure batch-to-batch consistency and reliability. The ACQUITY UPLC Glycoprotein BEH Amide Column is available in a variety of other combinations as well.

The ACQUITY UPLC Glycoprotein BEH Amide Column packing material is prepared in a cGMP, ISO 9001 certified facility using exceptionally pure reagents. Each batch of ACQUITY UPLC Glycoprotein BEH Amide Column is qualified using the Waters Standards, and the results are restricted to within specification ranges to ensure reproducible performance. Every column is also analyzed for efficiency, and an eCord containing a Certificate of Analysis and Performance Test Chromatogram is attached. Users who do not have an eCord reader but have a non-Waters LC system can access the same information.

The ACQUITY Amide columns are great for carbohydrate analysis and offer numerous advantages. The 1.7 m particle size allows for high-speed, high-resolution analysis in complicated matrices while maintaining or enhancing chromatographic resolution. The columns increase quantitation accuracy because, unlike amine-based columns, they are not vulnerable to Schiff base formation. The use of high pH and high temperature to collapse reducing sugar anomers, minimize analytical durations, and improve MS detection is possible due to their higher pH stability. The use of BEH technology in conjunction with a tri-functionally bound amide phase extends column life and increases test robustness.

To shop for lab equipment as per your laboratory needs, please check out our catalog or browse through our website. You will be able to evaluate all of Waters' products and services, as well as speak with one of our worldwide client representatives about any questions or problems you may have.

You might also want to check out Waters ACQUITY UPLC Peptide HSS T3 VanGuard Pre-column, 100Å, 1.8 µm, 2.1 mm X 5 mm, 1K - 15K, 3/pk; the ACQUITY Peptide VanGuard Pre-Columns are generally installed to protect the HSS T3 Columns as they are compatible with them.

What Is The Meaning Of The Phrase "Protein Expression"?

Protein expression encompasses the intricate processes involved in the generation, modification, and regulation of proteins within living organisms. This term can be used to denote either the specific proteins under investigation or the laboratory methodologies employed to produce proteins in protein research. It encompasses the diverse mechanisms by which proteins are synthesized, modified, and controlled, playing a crucial role in understanding their functions and studying their properties in both biological and experimental settings.