Chemistry |
Amide |
Separation Mode |
Hydrophilic Interaction (HILIC) |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
11 pH |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
150000 |
Particle Shape |
Spherical |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
130 Å |
QC Tested |
Glycan |
Format |
Column |
Surface Area |
185 |
System |
UHPLC |
Particle Technology |
BEH |
USP Classification |
L68 |
Inner Diameter |
3 mm |
Length |
75 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Application |
Glycan |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Glycan BEH Amide Column, 130Å, 2.5 µm, 3 mm X 75 mm, 1K - 150K, 1/pk
Depend on the Waters XBridge Glycan BEH Amide Column to be able to hold very polar substances, including carbohydrates and sugars. The lab equipment makes it simple to separate polar analytes across a range of characteristics, including polarity, structural moiety, and pKa. You can access a chemically stable, trifunctionally bonded amide phase that is stable across a wide pH range by using the XBridge Glycan BEH Amide Column.
The XBridge Glycan BEH Amide Column uses a chemically stable, trifunctionally bound amide phase and can provide exceptional stability and adaptability in HILIC separations lab equipment since it is based on Waters' unique ethylene bridged hybrid (BEH) particle. Waters invented this capability as a novel solution to the problem of maintaining and separating highly polar chemicals in HILIC stationary phases. Additionally, the XBridge Glycan BEH Amide Column shares the same selectivity as the ACQUITY UPLC BEH Amide columns, allowing researchers to transfer separations across HPLC and UPLC technology platforms with ease.
Each batch of the BEH Glycan material is specially quality-controlled using the 2-AB labeled Waters Glycan Performance to help ensure batch-to-batch uniformity and extremely identical separated glycan profiles. Additionally, chromatographers can now select the most suitable LC-based technology to satisfy their unique released glycan analysis application needs and lab equipment, ensuring that you get very similar results. The UPLC (i.e1.7 µm) and HPLC-based (2.5 µm XP and 3.5 µm) application needs are met by the three highly scalable particle sizes of the Waters BEH-based Glycan chemistry solutions. Our web store, which enables you to buy them directly from us, allows you to discover more about the lab equipment.
You may also want to learn more about the 2-AB Glycan Performance Test Standard. The components required to benchmark and assess ACQUITY UPLC BEH Glycan, 1.7 µm Columns are present in the 2-AB Glycan Performance Test Standard, which is 2-AB labeled Human IgG and has undergone quality control (QC) verification. It comes as a lyophilized powder in a Waters TruView vial. Total moles per vial: 228. Similar to the QCRM product line, it is advised that this standard be utilized frequently for testing column and system performance.
What Is The Molecular Weight Range Offered By The XBridge Glycan BEH Amide Column?
The Waters XBridge Glycan BEH Amide Column listed here is able to process molecular weights within the 1,000-150,000 range.
What Is The Maximum Pressure Recommended For The XBridge Glycan BEH Amide Column?
For this particular XBridge Glycan BEH Amide Column, the recommended maximum pressure is 18,000 psi (1240 Bar).