UPLC Column 2.1 x 150 mm

SKU: 186008471
ACQUITY UPLC Protein BEH SEC Column, 200 Å, 1.7 µm, 2.1 mm X 150 mm, 1/pk

Acquity UPLC Protein BEH SEC Column | 186008471

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Product Description

Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 200Å, 1.7 µm, 2.1 mm X 150 mm represents the first commercially available sub-2-µm SEC chemistry. Fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 10000 to 450,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods. Note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.

Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis

Note: If you are developing a new method, please consider our newer technology, ACQUITY Premier 250Å SEC Columns, for superior recovery, separation, and resolution.

Specifications

  • Chemistry

    SEC

  • Separation Mode

    SEC

  • Particle Substrate

    Hybrid

  • pH Range Min

    2.5 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    60 C

  • Maximum Pressure

    7000 psi (483 Bar)

  • Endcapped

    No

  • Bonding Technology

    SEC

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    10000

  • Molecular Weight Range Max

    450000

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    200 Å

  • QC Tested

    Protein

  • Format

    Column

  • Packing Solvent

    20% methanol in water

  • Surface Area

    220

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L33

  • Inner Diameter

    2.1 mm

  • Length

    150 mm

  • Carbon Load

    12 %

  • eCord

    Yes

  • UNSPSC

    41115709

  • Application

    Protein

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

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FAQs about ACQUITY UPLC Protein BEH SEC Column

What type of biomolecular characterization is the ACQUITY UPLC Protein BEH SEC Column specifically designed for?
The ACQUITY UPLC Protein BEH SEC Column is optimized for the separation of proteins and peptides based on size, making it suitable for characterizing protein complexes, aggregates, and fragments in detail.

Can ACQUITY UPLC Protein BEH SEC Column be utilized for quantitative protein analysis?
Yes, the ACQUITY UPLC Protein BEH SEC Column can be used for quantitative analysis as it provides consistent and reproducible retention times and peak areas, which are essential for quantitation.

How does temperature control affect the performance of the ACQUITY UPLC Protein BEH SEC Column?
Temperature control can significantly impact the performance of the ACQUITY UPLC Protein BEH SEC Column by influencing protein conformation and interaction with the stationary phase, thus affecting the resolution and reproducibility of separations.

Is the ACQUITY UPLC Protein BEH SEC Column compatible with a wide range of mobile phases for flexibility in protein separation?
Yes, the ACQUITY UPLC Protein BEH SEC Column is designed to be compatible with a variety of mobile phases, allowing for flexibility in optimizing protein separations according to specific research needs.

Are salt gradients applicable when using the ACQUITY UPLC Protein BEH SEC Column for protein analysis?
Typically, salt gradients are not used with the ACQUITY UPLC Protein BEH SEC Column as size exclusion chromatography relies on size-based separation, although certain conditions may warrant the use of salt to enhance protein recovery or stability.

How Do I Troubleshoot My ACQUITY UPLC Protein BEH SEC Column?

The first step is to compare the current column performance to performance when your column was last functioning properly. Functional tests with protein mixture may reveal subtle changes to surface chemistry that impact the application. Common symptoms of change to the column may include an increase in pressure, or a loss of resolution paired with increased peak tailing that may be caused by microbial contamination. Increased peak tailing can also be caused by a failure of a tubing connector or material build-up on the column inlet frit.