Line drawing of a column

SKU: 186008955
BioResolve RP mAb Polyphenyl Column, 450Å, 2.7 µm, 4.6 mm X 150 mm, 1/pk

BioResolve RP mAb Polyphenyl Column | 186008955


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Product Description

The first choice for reverse phase protein separation and analysis, BioResolve RP mAb Polyphenyl Column, 450Å, 2.7 µm, 4.6 mm X 150 mm Columns contain 2.7 µm superficially-porous-silica particles having an average pore diameter of 450Å. The particles are functionalized with a proprietary polyphenyl bonding process that was specifically designed for LC or LC-MS analyses of intact IgG monoclonal antibodies and their fragments. Each batch is qualified using a gradient separation of the Waters mAb Subunit Standard (p/n: 186008927).

Note p/n: 176004169 contains a BioResolve RP mAb Polyphenyl Column, 450Å, 2.7 µm, 4.6mm X 150mm, 1/pk with Intact mAb Mass Check Standard (p/n: 186006552) and mAb Subunit Standard (p/n: 186008927).

Suggested Use: Intact protein and mAb subunit analysis, Protein Analysis, ADC characterization, AAV, Antibody Conjugates

Specifications

  • Separation Mode

    Reversed Phase

  • Temperature Limits

    90 C

  • Maximum Pressure

    10000 psi (689 Bar)

  • Particle Size

    2.7 µm

  • Pore Size

    450 Å

  • QC Tested

    Protein

  • System

    HPLC, UHPLC, UPLC

  • USP Classification

    L11

  • UNSPSC

    41115709

  • Brand

    BioResolve

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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BioResolve RP mAb Polyphenyl Column, 450Å, 2.7 µm, 4.6 mm X 150 mm, 1/pk

The BioReserve RP mAb Polyphenyl Columns were created with the goal of performing high-quality reversed-phase chromatography (LC) or LC-MS analysis of complete monoclonal antibodies (mAbs), mAb subunits, and antibody-drug conjugates (ADCs). The utilization of silica-based solid core particles with a well-defined 450 pore covering and polyphenyl ligand bonding makes this achievable.

The BioResolve RP mAb Columns are made up of 2.7-m superficially porous silicon particles with a pore diameter of 450. The particles are functionalized using a unique polyphenyl bonding technique that was developed exclusively for LC or LC-MS analysis of entire IgG monoclonal antibodies and fragments. Note along with this particular column, a BioResolve RP mAb Polyphenyl Column, 450A, 2.7 um, 4.6mm X 150mm, 1/pkg with Intact mAb and mAb Subunit Standards is included in P/N 176004169.

Each batch of the BioReserve RP mAb Polyphenyl material is certified using a gradient separation of Waters mAb Subunit Standard, and the results are monitored and retained at a very limited specification range to assure column over column and batch over batch reproducibility. The packaging material for BioReserve RP mAb Polyphenyl Columns is made in the best world-class facilities under the most stringent conditions, ensuring the highest quality industry standards, including ISO 9001 and current Good Manufacturing Practices (CGMP).

Each unit of the BioReserve RP mAb Polyphenyl Columns is also efficiency tested, and an eCord with a Certificate of Analysis and Performance Test Chromatogram is attached. Users of non-Waters LC systems who do not have an eCord reader can still access this extensive information. The batch number, analysis of unbonded particles, analysis of bonded particles, and chromatographic results and conditions are all included in the Certificate of Analysis for each batch of packing material.

With the right lab equipment, you can boost your productivity. To discover more about the variations of the same product, such as the BioReserve RP mAb Polyphenyl Column, or to find more goods that work with it check out our shop for lab equipment; you may browse around our website, refer to our catalog and guarantee your lab needs are covered as needed.

You may also be interested in BioResolve RP mAb Polyphenyl VanGuard Cartridge, 450Å, 2.7 µm, 3.9 mm X 5 mm, 3/pk; This Part Number (SKU: 186008947) contains (3) VanGuard Cartridges. The VanGuard Universal Cartridge Holder (P/N 186007949) is required for use with these replaceable cartridges. PN 176004161 contains (3), 3.9 x 5mm VanGuard Cartridges as well as an included VanGuard Universal Cartridge Holder (P/N 186007949).

What Is HPLC (High-Performance Liquid Chromatography)?

High-performance liquid chromatography, or HPLC, is an analytical technique for separating, identifying, and quantifying components in a mixture. This test is used for a number of reasons and is the most widely utilized procedure in laboratories worldwide.