Line drawing of a column

SKU: 186008963
XBridge Oligonucleotide BEH C18 OBD Prep Column, 130Å, 2.5 µm, 30 mm X 50 mm, 1/pk

XBridge Oligonucleotide BEH C18 OBD Prep Column | 186008963


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Product Description

Waters Oligonucleotide Separation Technology columns are based on the second generation of hybrid-silica BEH Technology particles functionalized with C18. The separation of detritylated synthetic oligonucleotide samples is based on the well-established method of ion-pair, reversed-phase chromatography. The availability of 1.7µm UPLC technology or 2.5µm HPLC particles in various column dimensions provides flexibility to meet various laboratory-scale isolation or analysis needs and delivers exceptional sample resolution and superior column life. In addition, Waters manufacturing and quality control testing procedures help ensure consistent batch-to-batch and column-to-column performance regardless of application demands.

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Size

    2.5 µm

  • Pore Size

    130 Å

  • Format

    OBD Prep Column

  • Particle Technology

    BEH

  • USP Classification

    L1

  • Inner Diameter

    30 mm

  • Length

    50 mm

  • UNSPSC

    41115854

  • Application

    Oligonucleotide, Gene Therapeutics

  • Brand

    XBridge

  • Product Type

    Columns

  • Units per Package

    1 pk

Hazaradous Material This product contains hazardous material which requires special freight handling. Additional charges may apply.

Product Support

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XBridge Oligonucleotide BEH C18 OBD Prep Column, 130Å, 2.5 µm, 30 mm X 50 mm, 1/pk

The second generation of hybrid-silica BEH Technology particles functionalized with C18 are used in Waters Oligonucleotide Separation Technology columns. The well-established method of ion-pair, reversed-phase chromatography is used to separate detritylation synthetic oligonucleotide materials. The availability of 1.7m UPLC technology or 2.5m HPLC particles in various column dimensions allows for flexibility in meeting diverse laboratory-scale isolation or analysis needs while also delivering remarkable sample resolution and column life.

By utilizing the OBD design, which is resistant to poor solubility and pressure shocks associated with large volumes of pure organic solvent, the Waters XBridge Oligonucleotide BEH C18 OBD Prep Column delivers outstanding resistance to mechanical chromatographic bed failure. You also receive constant column-to-column performance, which lowers expenses by extending the lifetime of the columns. This is made feasible by employing ultra-pure reagents and adhering to the strictest manufacturing requirements, such as cGMP and ISO 9001:2000.

Increase your productivity with the Waters XBridge Oligonucleotide BEH C18 OBD Prep Column's higher recoveries. The lab equipment also has longer column lifetimes, which means fewer replacements and, as a result, lower expenses.

If you are interested in reviewing products that are compatible and similar in nature to the XBridge Oligonucleotide BEH C18 OBD Prep Column, including guards, vials, elution plates, we recommend you to check out our website or browse through our detailed brochure from where you can shop for lab equipment as per your needs.

We also recommend that you to review XBridge Oligonucleotide BEH C18 Method Validation Kit, 130Å, 2.5 µm, 4.6 mm X 50 mm, 3/pk; The reproducibility of a chromatographic column has a critical impact on the long-term reliability and robustness of an analytical method, and its reproducibility lies completely outside the user's control. With exceptional batch-to-batch and column-to-column reproducibility, Waters' well-established particle and column manufacturing process control provides confidence in the long-term reliability of your analytical method. ACQUITY OST C18, 135A, 2.5um Method Validation Kit includes three batches of chromatographic media [derived from different base particles] to judge the quality, reliability, and consistency of your chromatographic method. Each batch of selected ACQUITY OST C18, 135A, 2.5um chemistry is specifically QC tested with a mixture of synthetic oligonucleotides to help ensure consistent performance.

What Is The Meaning Of The Term Elution?

Elution, in the field of chromatography, refers to the procedure of extracting a substance that has been adsorbed onto a solid adsorbent by utilizing a solvent. It can also refer to the process of separating an antibody from the antigen it is bound to. The eluent or eluant acts as the "carrier" component of the mobile phase, facilitating the transport of analytes through the chromatograph during the elution process.