Note: This p/n: is also available with one vial the BEH200 SEC Protein Standard at no additional cost (p/n: 176004327).
Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis
Chemistry |
SEC |
Separation Mode |
Gel Filtration (aqueous) |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
6,500 psi (448 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
450000 |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
200 Å |
QC Tested |
Protein |
Format |
Online Column |
Packing Solvent |
20% methanol in water |
Surface Area |
220 |
System |
HPLC, UHPLC, UPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L20, L33, L59 |
Inner Diameter |
7.8 mm |
Length |
300 mm |
Carbon Load |
12 % |
eCord |
No |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Protein BEH SEC Column, 200Å, 2.5 µm, 7.8 mm X 300 mm, 1/pk
Invest in the XBridge Protein BEH SEC, 200 Column, which features 2.5um particles of Waters' Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating used in our successful series of HPLC-based SEC columns.
With the XBridge Protein BEH SEC Column, you can easily design and transfer SEC procedures based on laboratory apparatus. The lab equipment also allows you to create methods based on the resolution of required protein components and sample throughput requirements. The XBridge Protein BEH SEC Columns were developed specifically for size-exclusion chromatography of proteins to complement Waters' existing line of smaller particle size UPLC-based SEC columns.
The XBridge Protein BEH SEC Column offers a longer column life than silica-based SEC columns because it has fewer unwanted protein-column interactions. The lab equipment also provides better throughput and delivers HPLC-based SEC resolution of proteins from 10,000 to 450,000 Daltons. The flow and pressure tolerance of these diol-bonded XBridge Protein BEH SEC Columns allows for enhanced sample throughput on HPLC equipment. They deliver exceptional stability, performance, and lifetime by combining BEH base particle technology with new diol bonding techniques.
The XBridge Protein BEH SEC Column, like all lab equipment developed and delivered by Waters, ensures consistent and reliable performance because it is manufactured in cGMP, ISO9001, and ISO13485 certified facilities. Comprehensive testing is also performed at each stage, from packing materials to post-production, to ensure unrivaled column consistency and your confidence in validation techniques.
When compared to silica-based SEC columns, the XBridge Protein BEH SEC Column has fewer unwanted protein-column interactions. This allows the column to have a long service life. The diol-bonded XBridge Protein BEH SEC Column allows for higher sample throughput on HPLC systems while providing for flow and pressure tolerance. Column stability, performance, and lifetime can all be achieved by combining BEH base particle technology with new diol bonding techniques.
We recommend that you also explore and invest in the BEH200 SEC Protein Standard Mix. The Standard Mix is a 5 component protein mixture (Thyroglobulin, IgG, BSA, Myoglobin, Uracil) used to benchmark analytical columns.
Is There An Operating pH Limit For The XBridge Protein BEH SEC Column?
The XBridge Protein BEH SEC Column listed here performs well within the operating pH range of 2.5-8.