The BioResolve SEC mAb Guard is specifically designed to extend the life of the BioResolve SEC mAb Columns. It effectively traps inadvertent injection of insoluble particulates and/or excipients sometimes present in samples and/or SEC eluents. The BioResolve SEC mAb Guard will not degrade the separation of mAb aggregates.
Chemistry |
SEC |
Separation Mode |
SEC |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
4500 psi (310 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
450000 |
Particle Shape |
Spherical |
Particle Size |
2.5 µm |
Endfitting Type |
Waters |
Pore Size |
200 Å |
QC Tested |
Protein |
Format |
Column |
Packing Solvent |
10% acetonitrile/90% 25 mM sodium phosphate pH 7.0 + 100 mM KCl |
Surface Area |
220 |
System |
HPLC, UHPLC, UPLC |
Particle Technology |
BEH |
Technique |
SEC |
USP Classification |
L20, L33, L59 |
Inner Diameter |
4.6 mm |
Length |
150 mm |
Carbon Load |
12 % |
eCord |
Yes |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
BioResolve |
Product Type |
Columns |
Units per Package |
1 pk |
BioResolve SEC mAb Column, 200Å, 2.5 µm, 4.6 x 150 mm, 1/pk
Accurate charge variant quantitation of monoclonal antibodies requires a separation tool that’s ready to help you overcome analytical challenges, and the BioResolve SEC mAb Column is here to do exactly that and so much more. This mAb column comes outfitted with the Waters mAb Size Variant Standard at no extra charge, so you can verify LC-MS instrument performance before performing separations. What’s more, this breakthrough column offers a wide and usable pH range that can accommodate various analytes. Add the BioResolve SEC mAb Column to your lab’s toolkit and quantify monoclonal antibodies successfully today.
How is the BioResolve SEC mAb Column so unique?
For one, it’s specifically designed to ensure accurate and reproducible quantitation of monoclonal antibody monomers. But even more, it’s also designed to provide that quantitation from both high and lower molecular weight fragments. And to ensure batch-to-batch consistency, each and every BioResolve SEC mAb Column must undergo a strict QC process before shipping out to you. This is how we only provide the proper working instruments right out of the box.
Speaking of working right out of the box, which LC-MS systems can the BioResolve SEC mAb Column work with?
This column is LC-MS system agnostic, which means you can use it with HPLC, UHPLC and UPLC systems. This especially comes in handy when you’re a scientists who wants to work with the separation speed, sensitivity and power of UPLC systems. But if you’re developing methods, this mAb column is a great tool to use because of its flexible system compatibility.
Protecting your BioResolve SEC mAb Column from contaminants should be a huge priority whenever you’re in the lab. Although proper sample purifications protocols remove many of the contaminants in the sample, there could be miniscule contaminants flowing in the mobile phase stream. To meet that concern head-on, we’re happy to provide the BioResolve SEC mAb Guard. This guard column removes contaminants from the mobile phase stream before they reach your precious analytical column.
We have so much more than just mAb columns for proper mAb quantitation at Waters as well. From full LC-MS systems and quality control reference materials to sample prep solution kits and so much more, we truly have it all. Shop lab equipment and transform your experiments today.
What’s the BioResolve SEC mAb Column’s Maximum Pressure Limit?
The BioResolve SEC mAb Column can withstand pressures up to 4,500 PSI (310 Bar).