BioResolve SEC Column 4.6x300mm angle right

SKU: 186009437
BioResolve SEC mAb Column, 200Å, 2.5 µm, 4.6 x 300 mm, 1/pk

BioResolve SEC mAb Column | 186009437

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Product Description

Primarily used for legacy methods. If you are developing a new method, please consider our newer technology XBridge Premier Protein 250Å SEC and ACQUITY Premier Protein 250Å SEC Columns for superior recovery, separation, and resolution.

The BioResolve SEC mAb Guard is specifically designed to extend the life of the BioResolve SEC mAb Columns. It effectively traps inadvertent injection of insoluble particulates and/or excipients sometimes present in samples and/or SEC eluents. The BioResolve SEC mAb Guard will not degrade the separation of mAb aggregates.

Specifications

  • Chemistry

    SEC

  • Separation Mode

    SEC

  • Particle Substrate

    Hybrid

  • pH Range Min

    2.5 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    60 C

  • Maximum Pressure

    6,500 psi (448 Bar)

  • Endcapped

    No

  • Bonding Technology

    Diol (OH)

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    10000

  • Molecular Weight Range Max

    450000

  • Particle Shape

    Spherical

  • Particle Size

    2.5 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    200 Å

  • QC Tested

    Protein

  • Format

    Online Column

  • Packing Solvent

    10% acetonitrile/90% 25 mM sodium phosphate pH 7.0 + 100 mM KCl

  • Surface Area

    220

  • System

    HPLC, UHPLC, UPLC

  • Particle Technology

    BEH

  • Technique

    LC

  • USP Classification

    L20, L33, L59

  • Inner Diameter

    4.6 mm

  • Length

    300 mm

  • Carbon Load

    12 %

  • eCord

    Yes

  • UNSPSC

    41115711

  • Application

    Protein

  • Brand

    BioResolve

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

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BioResolve SEC mAb Column, 200Å, 2.5 µm, 4.6 x 300 mm, 1/pk

Biological separation challenges are aplenty, and they grow as science morphs and progresses, so don’t you want a separation tool that can rise to meet those challenges?
We’re proud to present the BioResolve SEC mAb Column, designed specifically for the quantitation of monoclonal antibody monomers. And because we care how your LC-MS system performs with mAb analysis assays, we’ve included the Waters mAb Size Variant Standard free of charge. This standard can be used to ensure that your LC-MS system is operating as it should. Add the BioResolve SEC mAb Column to your lab’s toolkit to improve your mAb analyses for the better!

A huge benefit to owning the BioResolve SEC mAb Column is that it can be used on HPLC, UPLC and UHPLC systems. This kind of flexibility makes this column highly desirable by scientists across the globe, and for good reason. This column is equipped with features to help you overcome the analytical challenges inherent in the analysis of mAb-based therapeutic drugs. Plus, each and every BioResolve SEC mAb Column must undergo strict QC testing with the mAb Size Variant Standard before being delivered to you. This helps us to ensure that you only get the best-performing products right out of the box.

Speaking of analytical challenges, what about particulate contamination of the column?
While sample preparation protocols should be closely followed so you only run purified samples through your column, there’s still a chance the mobile phase stream has small particulate contaminants in it. To overcome that challenge, we’re happy to provide the BioResolve SEC mAb Guard. This guard seamlessly incorporates into your existing workflow to provide enhanced protection, leading to longer and healthier column lifetimes.

BioResolve Columns are often characterized by providing the features needed to overcome analytical challenges in therapeutic drug analysis. And with multiple column chemistries and separation modes offered, you’re sure to find the right mAb column for your separations. You need only browse the entire BioResolve Column family for the exact column configuration you need.

At Waters, we have much more than just BioResolve Columns. With LC-MS systems to push your lab into the future, quality control reference materials for benchmarking purposes and lab informatics to compile and analyze your data at scale, we truly have everything you need to be successful in the lab. Shop lab equipment to see our entire selection of products and resources.

What’s the BioResolve SEC mAB Column’s Separation Technology?

The BioResolve SEC mAB Column offers Size-Exclusion Chromatograph (SEC) separation technology.

What Are The Temperature Limits Of The BioResolve SEC mAb Column?

This particular BioResolve SEC mAb Column offers a maximum temperature of 60 C. It is important to note that the use of different pressures and buffers at different pH can also impact the temperature limit while reducing the lifetime of the column.