The BioResolve SEC mAb Guard is specifically designed to extend the life of the BioResolve SEC mAb Columns. It effectively traps inadvertent injection of insoluble particulates and/or excipients sometimes present in samples and/or SEC eluents. The BioResolve SEC mAb Guard will not degrade the separation of mAb aggregates.
Chemistry |
SEC |
Separation Mode |
SEC |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
4500 psi (310 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
450000 |
Particle Shape |
Spherical |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
200 Å |
QC Tested |
Protein |
Format |
Guard Column |
Packing Solvent |
10% acetonitrile/90% 25 mM sodium phosphate pH 7.0 + 100 mM KCl |
Surface Area |
220 |
System |
HPLC, UHPLC, UPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L20, L33, L59 |
Inner Diameter |
4.6 mm |
Length |
30 mm |
Carbon Load |
12 % |
eCord |
No |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
BioResolve |
Product Type |
Guards |
Units per Package |
1 pk |
BioResolve SEC mAb Column with BioResolve SEC mAb Guard, 200Å, 2.5 µm, 7.8 x 150 mm, 1/pk with mAb Size Variant Standard
Protecting your BioResolve SEC mAb Column from particulate contaminants should always be at the forefront of your mind when using this column in the lab. Although proper sample purification protocols remove a lot of the concern around contamination, they do not account for contaminants that may be lingering in the mobile phase stream. But with the BioResolve SEC mAb Guard, you can better protect your BioResolve Column from those miniscule contaminants. This guard column actively traps insoluble particles, removing them from the workflow before they reach your column. Add the BioResolve SEC mAb Column to your analytical workflow now and you’ll be ready to enjoy an improved overall column lifetime.
The BioResolve SEC mAb Guard upholds the mechanical and chemical stability needed to properly perform monoclonal antibody analysis. That’s achieved by the guard column offering the same diameter as the inner lining of the column itself, plus the guard is packed with the same sorbent as the BioResolve SEC mAb Column. Worried about how adding an extra step may degrade the separation of mAb aggregates?
Your concerns can be put to rest, as the BioResolve SEC mAb Guard easily overcomes any of those degradation concerns.
How does the BioResolve SEC mAb Guard work?
This guard column incorporates seamlessly into your existing workflow. Place between the analytical column and mobile phase injector, the guard acts as an extra step for the mobile phase stream to flow through. As the stream flows through, the guard picks up any contaminants and traps them before they enter your column. This helps reduce the need for constant reproducibility, leading to a more efficient and happier lab for all. What’s more, when the BioResolve SEC mAb Guard becomes worn and needs to be replaced, you can do so with another genuine replacement from Waters Corporation.
Before pursuing any mAb separation, it’s important to make sure your LC-MS system is operating as it should with regard to selectivity. To make system benchmarking easier, we’re proud to provide the a href="https://www.waters.com/nextgen/us/en/shop/standards--reagents/186009429-mab-size-variant-standard.html">mAb Size Variant Standard. This standard can be used with any LC-MS system.
Once you’re done adding all the essentials to your cart, make sure to check out all of the other incredible products and resources available at Waters Corporation. Shop lab equipment to see how our products and resources can help push your lab into the future.
What’s the BioResolve SEC mAb Guard’s pH Range?
The BioResolve SEC mAb Guard offers a range of 2.5 to 8 pH.