ACQUITY PREMIER Peptide BEH C18 Column 130Å 186009483 angle right

SKU: 186009483
ACQUITY Premier Peptide BEH C18 Column, 130Å, 1.7 µm, 2.1 x 150 mm, 1/pk

ACQUITY Premier Peptide BEH C18 Column | 186009483

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Product Description

The ACQUITY Premier Peptide BEH C18, 130Å, 1.7 µm 2.1 x 150 mm Peptide Separation Technology Columns have been specially optimized and QC tested with cytochrome c digest standards (p/n: 186006371) for peptide separations. The small-pore (130Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis. 2.1mm ID columns are recommended for use with low dispersion (UPLC or UHPLC) systems, especially for LC-MS methods. This column is part of the Premier class of columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes.

Suggested Use: Peptide Separations, Peptide Analysis, Peptide Mapping

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    1 pH

  • pH Range Max

    12 pH

  • Temperature Limits

    80 C

  • Maximum Pressure

    18000 psi (1240 Bar)

  • Endcapped

    Yes

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    130 Å

  • QC Tested

    Peptide

  • Format

    Premier Column

  • Surface Area

    185

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • Technique

    LC

  • USP Classification

    L1

  • Inner Diameter

    2.1 mm

  • Length

    150 mm

  • Carbon Load

    18 %

  • eCord

    Yes

  • UNSPSC

    41115711

  • Application

    Peptide

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

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ACQUITY Premier Peptide BEH C18 Column, 130Å, 1.7 µm, 2.1 x 150 mm, 1/pk

Maximize throughput and increase reproducibility of desired results with the ACQUITY Premier Peptide BEH C18 Column. Ethylene-bridged hybrid (BEH) technology is built into every one of these UPLC columns because of the terrific peak shape it can offer scientists working under reverse-phase liquid chromatographic conditions. Even more, this product offers ultra-low column bleed, resulting in data you can actually trust and reproduce easily.

Peptide synthesis and characterization is one of the biggest obstacles put in front of scientists today. But with the ACQUITY Premier Peptide BEH C18 Column, that obstacle is never a worry. That’s because this column offers a wide pH range and outstanding temperature stability in peptide mapping, proteomics and other peptide-focused assays. Plus, with a low-positive-charged surface on the column, TFA-containing eluents can be used.

Are peptide separations by UPLC your goal?
The separation of peptides can sometimes be difficult, but it doesn’t have to be with the ACQUITY Premier Peptide BEH C18 Column. This column eliminates the need to compromise between a traditional reversed-phase eluent that delivers sharp peaks and one that reduces the MS signal, too. Scientists across the globe love this kind of versatility and reliability, especially since these factors can lead to increase business productivity and profitability.

While the UPLC application is demanding, this UPLC component is designed to provide superior resolution that’s easily reproducible. But to make sure your column lasts across many successful assays, we recommend you buy an ACQUITY Premier BEH C18 VanGuard FIT Cartridge to extend column lifetime. This helpful tool simply threads into your existing column and protects it from sample and matrix contaminants. That kind of protection is at the forefront of Waters products

This UPLC column also belongs to the MaxPeak Premier classification, which is reserved for LC products delivering premium chromatographic performance while working hard to reduce any unwanted analyte-surface interactions. This especially comes in handy during protein characterization, as reversed-phase chromatography can be demanding and cause stress on any involved products.

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What’s the Maximum Pressure Allowed by the ACQUITY Premier Peptide BEH C18 Column?

The ACQUITY Premier Peptide BEH C18 Column can withstand pressures of up to 18,000 PSI, but of course we recommend you subject this column to pressures below that threshold.