ACQUITY Premier Peptide BEH C18 300 Å Column 186009493 angle right

SKU: 186009493
ACQUITY Premier Peptide BEH C18 Column, 300Å, 1.7 µm, 2.1 x 50 mm, 1/pk

ACQUITY Premier Peptide BEH C18 Column | 186009493


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Product Description

The ACQUITY Premier Peptide BEH C18 Column, 300Å, 1.7 µm, 2.1 mm X 50 mm Peptide Separation Technology Columns have been specially optimized and QC tested with cytochrome c digest standards (p/n: 186006371) for peptide separations. The wide-pore (300Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for especially large peptides in proteomics, protein characterization, and peptide synthesis. 2.1mm ID columns are recommended for use with low dispersion (UPLC or UHPLC) systems, especially for LC-MS methods. This column is part of the MaxPeak Premier Class of Columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes.

Suggested Use: Peptide Separations, Peptide Analysis

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    1 pH

  • pH Range Max

    12 pH

  • Temperature Limits

    80 C

  • Maximum Pressure

    18000 psi (1240 Bar)

  • Endcapped

    Yes

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    300 Å

  • QC Tested

    Peptide

  • Format

    Premier Column

  • Surface Area

    185

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • Technique

    LC

  • USP Classification

    L1

  • Inner Diameter

    2.1 mm

  • Length

    50 mm

  • Carbon Load

    18 %

  • eCord

    Yes

  • UNSPSC

    41115711

  • Application

    Peptide

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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ACQUITY Premier Peptide BEH C18 Column, 300Å, 1.7 µm, 2.1 x 50 mm, 1/pk

To obtain peptide separations that meet the application requirements of UPLC systems, you need a strong and flexible analytical column that’s truly up for the challenge. That’s where the ACQUITY Premier Peptide BEH C18 Column comes into play. This incredible reverse-phase peptide column offers a trifunctionally bonded Ethylene-Bridged Hybrid (BEH) base that promotes a wide pH range to accommodate varying analytes, further proving that this column is an essential tool to have during proteomics or peptide mapping-related assays. Plus, with MaxPeak High-Performance Surface technology protecting your analytical workflow from unwanted analyte-surface interactions, you can easily pave a path toward MS adoption when you have the ACQUITY Premier Peptide BEH C18 Column at your command.

At the heart of every ACQUITY Premier Peptide BEH C18 Column is the trifunctionally bonded Ethylene-Bridged Hybrid (BEH) base particle. It’s worth noting that because of this state-of-the-art base particle, this analytical column offers superior stability when dealing with low-pH mobile phases. And because your important samples are always prioritized by our products, know that this column also exhibits ultra-low bleed, which is perfect when MS adoption is taken into consideration. Using high temperatures or backpressures?
The ACQUITY Premier Peptide BEH C18 Column has the mechanical stability to withstand those factors.

Although peptide reverse-phase columns are usually characterized by their ability to deliver highly resolved separations of complex peptide mixtures, the ACQUITY Premier Peptide BEH C18 Column takes that a step further. How?
For one thing, the BEH particle included offers the highest stability of any chromatographic particle. And in addition to all that, that hybrid particle makes it easier to control silanol activity during your assays. This especially helps with gaining better data reproducibility

Not performing peptide-related assays but want the separation performance offered by ACQUITY Columns?
Shop ACQUITY Columns now to find the right match for your assays. Don’t forget to tell your lab manager all about the incredible separation technology offered by our UPLC columns.

To keep your lab running efficiently, you need specialty products, equipment and resources immediately within reach. Luckily for you, we have everything you need to be successful in the lab. Shop for lab equipment now to explore everything we have to offer.

What Are The Temperature Limits Of The ACQUITY Premier Peptide BEH C18 Column?

The ACQUITY Premier Peptide BEH C18 Column listed here can work with 60 C (Low pH) / 60 C (High pH).