Suggested Use: Peptide Separations, Peptide Analysis
Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
12 pH |
Temperature Limits |
80 C |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
300 Å |
QC Tested |
Peptide |
Format |
Premier Column |
Surface Area |
185 |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L1 |
Inner Diameter |
2.1 mm |
Length |
150 mm |
Carbon Load |
18 % |
eCord |
Yes |
UNSPSC |
41115711 |
Application |
Peptide |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY Premier Peptide BEH C18 Column, 300Å, 1.7 µm, 2.1 x 150 mm, 1/pk
Prioritize speed and resolution in peptide separations with the ACQUITY Premier Peptide BEH C18 Column. Thanks to the Ethylene-Bridged Hybrid (BEH) particle technology in this silica column, you can perform size-exclusionary or reverse-phase chromatographic separations and get terrific peak shape delivered right back. And because data reproducibility is of the utmost importance when you’re hard at work in the lab, this column provides ultra-low bleed so you can remain confident in your data. What’s more, the ACQUITY Premier Peptide BEH C18 Column has earned the MaxPeak designation, which means this UPLC column employs breakthrough technology to reduce unwanted analyte-surface interactions.
Proteomics by nature presents a series of obstacles that even the most experienced scientists will have to overcome. That’s why the ACQUITY Premier Peptide BEH C18 Column was designed specifically for those assays and others involving peptide separations, including protein characterization. But even more than that, this UPLC column provides outstanding mechanical stability at increased temperatures needed for peptide mapping, and it offers a wide pH range to accommodate various analytes. Add in the MaxPeak High-Performance Surface technology and it’s easy to see why so many scientists turn to this product for protein analysis.
Need to use TFA-containing eluents?
Thanks to the low-positive-charged surface contained in this column, you can do exactly that. But that’s not the only flexibilities offered by the ACQUITY Premier Peptide BEH C18 Column, as this UPLC tool provides incredible chemical stability. Couple that with the small pore size and increased resolution compared to other columns and it becomes immediately apparent why ACQUITY Premier Columns exist at the forefront of the market.
Even if you’re not performing proteomics-related assays, you’ll want the speed and increased resolution offered by ACQUITY Premier Columns. With 8 different UPLC particles of various substrate technologies available, you’re sure to find a silica column that’s right for your application. Shop ACQUITY Columns to find the right match, or tell your lab manager today that you want an ACQUITY Column at your disposal.
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What Is A Sample Matrix?
A sample matrix is used to describe everything that is present in the typical sample except for the analytes of interest.