Suggested use: Glycan Analysis, Glycopeptide analysis
Chemistry |
Amide |
Separation Mode |
Hydrophilic Interaction (HILIC) |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
11 pH |
Temperature Limits |
90 C |
Maximum Pressure |
15000 psi (1034 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
130 Å |
QC Tested |
Glycan |
Format |
Premier Column |
Surface Area |
185 |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
Technique |
LC/MS |
USP Classification |
L68 |
Inner Diameter |
2.1 mm |
Length |
150 mm |
Carbon Load |
12 % |
eCord |
Yes |
UNSPSC |
41115709 |
Application |
Glycan |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY Premier Glycan BEH Amide Column, 130Å, 1.7 µm, 2.1 x 150 mm Column, 1/pk
Get reliable and repeatable results for glycan analysis with the ACQUITY Premier Glycan Column. This HILIC amide column takes hybrid-silica BEH technology particles and an amide-containing species to promote high resolving power in all related assays. And unique to Waters columns, it offers MaxPeak High Performance Surface technology to help reduce unwanted analyte and surface interactions, resulting in more reliable data and peak performance for when you need it most.
This ACQUITY UPLC BEH Amide Column is designed for HILIC-mode separations of 2-aminobenzamide (2-AB), 2-aminobenzoic acid (2-AA) or Waters RapiFluor-MS (RFMS) labeled glycans. The glycan material contained in this column is also tested for quality control by Waters Glycan Performance standards in order to ensure consistency across all BEH amide columns in this product line.
Did you know that this HILIC amide column is a terrific tool for carbohydrate analysis?
That’s because the small particle size enables high resolution in complex sample matrices while improving chromatographic resolution. Even more, this column for glycan analysis is not susceptible to Schiff base formation, which helps to improve quantitation accuracy. This makes the ACQUITY Premier Glycan BEH Amide Column an essential piece of lab equipment for the analysis of N- and O-linked glycans
Stability and versatility in HILIC separations is a must, which is why the ACQUITY Premier Glycan BEH Amide Column has a trifunctionally-bonded amide phase to ensure repeatable results across varying experiments. This amide column chemistry is also incredibly useful in retaining polar analytes that are too polar for reverse-phase chromatography. In addition, it offers orthogonal selectivity when compared to reversed-phase materials, making this HILIC column an alternative separation technique you can really trust.
Sample preparation can be time consuming when considering glycan analysis, but it doesn’t have to be anymore with the Glycoworks RapiFluor-MS N-Glycan Kit. This kit comes with RapiFluor, which has unique chemical attributes to help provide increased fluorescence quantification and quicker mass spectral response, making your glycan analysis assays both less complicated and easier to streamline.
All of the Waters columns for sale are on the cutting edge of technology and liquid chromatography. But that’s not the only lab products we manufacture to help scientists everywhere achieve reliable results. Shop for lab equipment today to find all the items your lab requires to operate successfully, including but not limited to sample collection plates, mass spectrometry equipment and even standards and reagents for you to reference during your assays.
What Compounds Can the ACQUITY Premier Glycan BEH Amide Column Retain?
The ACQUITY Premier Glycan BEH Amide Column can help analyze a wide variety of compounds, although it’s specifically designed to retain very polar compounds, including carbohydrates and sugars.