Suggested Use: Glycoprotein analysis, Glycopeptide analysis
Chemistry |
Amide |
Separation Mode |
Hydrophilic Interaction (HILIC) |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
10 pH |
Temperature Limits |
90 C |
Maximum Pressure |
15000 psi (1034 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
300 Å |
QC Tested |
Glycoprotein |
Format |
Premier Column |
Surface Area |
185 |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
Technique |
LC/MS |
USP Classification |
L68 |
Inner Diameter |
2.1 mm |
Length |
50 mm |
Carbon Load |
12 % |
eCord |
Yes |
UNSPSC |
41115709 |
Application |
Glycan |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY Premier Glycoprotein BEH Amide, 300Å, 1.7 µm, 2.1 x 50 mm Column, 1/pk
Tackle complicated glycopeptide and glycoprotein analysis with the efficiency, flexibility and reliability offered by the ACQUITY Premier Glycoprotein BEH Amide Column. This is more than just a UPLC silica column, as it has been professionally tested with Waters Glycoprotein Performance Test Standard to ensure premium performance right out of the box, plus MaxPeak High Performance Surface Technology to greatly reduce unwanted analyte-surface interactions. What’s more, the sub-2µm particle technology maximizes separation performance when you need it most. It should come as no surprise that the ACQUITY Premier Glycoprotein BEH Amide Column is a top choice among scientists who perform deglycosylation-related assays.
Hydrophilic Interaction Chromatography by nature is difficult and requires a separation tool that prioritizes retention time and peak response. This is in part due to difficult samples being used during related assays. That’s why we’re proud to provide this BEH amide silica column, which incorporates easily with HILIC method development and is sure to help you pave a path toward MS adoption without the usual pitfalls inherent in deglycosylation.
When separation performance is important, the ACQUITY Premier Column family is here to satisfy. This column group is filled with consumables that work well with UPLC systems, especially when varying flow rates and linear velocities are being used. No matter if you need a tool for glycan analysis or oligonucleotide separations, there’s an ACQUITY Premier Column that’s suited for the job.
Want to protect the ACQUITY Premier Glycoprotein BEH Amide Column from chemical or particulate fouling across multiple assays?
Buy a ACQUITY Premier BEH Amide VanGuard FIT Cartridge and you’ll have supreme confidence in your data when you use this column multiple times. VanGuard FIT technology easily incorporates into your analytical column to prevent losses in signal intensity. All adding up to a tool you can rely on time and again.
Paving a path toward MS adoption is only one thing we help scientists do at Waters with our breakthrough lab products. We also carry lab informatics, software, quality control reference materials and sample preparation products. Shop for lab equipment or tell your lab manager that you want Waters products in your toolkit.
What Is a HILIC Amide Column?
A HILIC Amide Column is an analytical column used in Hydrophilic Interaction Chromatography and is characterized by a stable, amide-containing species.