Suggested Use: Sialylated Glycan analysis, Glycan Analysis
Chemistry |
RP/Anion-exchange |
Separation Mode |
Mixed mode |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
10 pH |
Temperature Limits |
90 C |
Maximum Pressure |
15000 psi (1034 Bar) |
Endcapped |
Yes |
Bonding Technology |
C18 AX |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
95 Å |
QC Tested |
Glycan |
Format |
Premier Column |
Surface Area |
270 |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
Technique |
LC, LC/MS |
USP Classification |
L78 |
Inner Diameter |
2.1 mm |
Length |
50 mm |
Carbon Load |
17 % |
eCord |
Yes |
UNSPSC |
41115709 |
Application |
Glycan |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY Premier Glycan BEH C18 AX, 95Å, 1.7 µm, 2.1 x 50 mm Column, 1/pk
Glycan analysis by HPLC can present a series of difficult obstacles even for the most talented scientists, but with the ACQUITY Premier Glycan BEH C18 AX Column, overcoming those obstacles is a certainty. This anion-exchange column is designed to provide superior HPLC resolution for acidic glycans. And since this BEH C18 column comes protected by MaxPeak High Performance Surface Technology, you can tackle complicated glycan separations and analyses with minimal concern for unwanted surface-analyte interactions.
To create a charge-based solution for glycan analysis, the ACQUITY Premier Glycan BEH C18 AX Column incorporates an MS-grade mobile-phase concentrate and the RapiFluor-MS Sialylated Glycan Performance Test Standard. This way you can have an all-in-one solution for all glycan-related HPLC assays. Charge-based HPLC separations may be unwieldy to some products, but Waters scientists went back to the lab to make sure scientists everywhere can benefit from the breakthrough technology this column exhibits.
Want to protect your anion-exchange column and increase its lifetime?
Buy an ACQUITY Premier Glycan BEH C18 AX VanGuard FIT Cartridge and you’ll be ready to tackle multiple assays without losses in signal intensity. Every VanGuard FIT Cartridge provides superior HPLC column protection through active prevention of matrix and sample contaminants. This is especially important during glycan analysis, as acidic glycans can sometimes be tough to work with and separate properly. Plus, this HPLC column guard just threads onto the analytical column itself. Easy installation and superior protection; it’s what we care about at Waters.
Due to the nature of acidic glycans, their propensity can often be lost because of metallic surfaces. That’s why the ACQUITY Premier Glycan BEH C18 AX Column incorporates MaxPeak High Performance Surface Technology to prevent any of those unwanted interactions that may result in sample loss or losses in signal intensity. The barrier layer inside this HPLC column removes the concern about unwanted secondary interactions, which results in reliable and reproducible data. This in turn can lead to increased lab efficiency and business profitability.
At Waters, we strive to provide all the equipment that scientists need to complete complicated LC-MS assays. From HPLC systems to chromatography consumables and quality control reference materials, we simply have everything you need. Shop lab equipment now to be ready for the scientific future of tomorrow.
What Is an Anion-Exchange Column?
An anion-exchange column is a liquid chromatography product used specifically to separate and analyze charged molecules, like acidic glycans.