Suggested Use: Sialylated Glycan analysis, Glycan Analysis
Chemistry |
RP/Anion-exchange |
Separation Mode |
Mixed mode |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
10 pH |
Temperature Limits |
90 C |
Maximum Pressure |
15000 psi (1034 Bar) |
Endcapped |
Yes |
Bonding Technology |
C18 AX |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
95 Å |
QC Tested |
Glycan |
Format |
Premier Column |
Surface Area |
270 |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
Technique |
LC, LC/MS |
USP Classification |
L78 |
Inner Diameter |
2.1 mm |
Length |
150 mm |
Carbon Load |
17 % |
eCord |
Yes |
UNSPSC |
41115709 |
Application |
Glycan |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY Premier Glycan BEH C18 AX, 95Å, 1.7 µm 2.1 x 150 mm Column, 1/pk
Not just any UPLC column can handle glycan separations while offering superior resolution, but the ACQUITY Premier Glycan BEH C18 AX Column can do that and so much more. Incorporating state-of-the-art Ethylene-Bridged Hybrid (BEH) particle technology and anion-exchange features, this silica column can help you conquer all the obstacles inherent in separating acidic glycans using UPLC systems. What’s more, this column offers MaxPeak High-Performance Surface technology to help prevent unwanted analyte-surface interactions from negatively affecting your data. Buy the ACQUITY Premier Glycan BEH C18 AX Column now or tell your lab manager that you want ACQUITY Premier Column technology in your toolkit.
To ensure that this UPLC column is ready for difficult glycan separations and analysis, Waters scientists added a MS-grade mobile-phase concentrate and Rapifluor-MS Sialylated Glycan performance test standard that provides a charged-base solution. This not only helps to increase analyte recovery and data reproducibility, it can help you pave a successful path toward MS adoption. Add in the MaxPeak High-Performance Surface technology and it’s easy to see how the ACQUITY Premier Glycan BEH C18 AX Column is instilling confidence in scientists across the globe.
How can this anion-exchange column produce such great selectivity for acidic glycans?
Thanks to the charge-based separation solution, of course. The Ethylene-Bridged Hybrid (BEH) particle substrate also helps to boost analyte peak shape, efficiency and chemical stability despite all the obstacles inherent in glycan analysis. And with a wide pH range, you can use varying analytes without negatively affecting separation performance or speed.
Glycan analysis columns are only one of the many ACQUITY Premier Columns available. ACQUITY Columns are characterized by improving speed, sensitivity and resolution in methods development. With 8 UPLC particles of different substrate technologies and 22 stationary phases available, you’re sure to find an ACQUITY Column that matches your assays. Shop ACQUITY Columns today or tell your lab manager to stock your toolkit with ACQUITY technology now.
It’s not just our passion to provide quality lab materials and equipment to scientists aross the world, it’s also what drives us to improve. As science changes rapidly, so should our products to reflect that change and adapt to new environments. No matter if you need lab informatics, quality control reference materials or elution plates for sample preparation, we have what you desire. Shop lab equipment to discover all we have to offer.
What is the pH Range of the ACQUITY Premier Glycan BEH C18 AX Column?
The ACQUITY Premier BEH C18 AX Column offers a pH range of 2 to 10 pH.