ACQUITY Premier Peptide CSH C18 Column 130Å 186009488 angle right

SKU: 186009840
XSelect Premier Peptide HSS T3 Column, 100 Å, 2.5 µm, 2.1 x 100 mm

XSelect Premier Peptide HSS T3 Column | 186009840


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Product Description

The XSelect Premier Peptide HSS T3 Column, 100 Å, 2.5 µm, 2.1 x 100 mm Columns are designed for separations where silica-based selectivities are desired or when increased retention of hydrophilic peptides are required. Each batch of HSS T3, 100Å material is QC tested with a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) and must pass stringent performance criteria before acceptance for use in these columns. 2.1mm ID columns are recommended for use with low dispersion (UPLC or UHPLC) systems, especially for LC-MS methods. This column is part of the MaxPeak Premier class of columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes.

Suggested Use: Peptide Separations, Peptide Analysis

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Silica

  • pH Range Min

    2 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    45 C

  • Maximum Pressure

    18000 psi (1240 Bar)

  • Endcapped

    Yes

  • Bonding Technology

    T3

  • Silanol Activity

    Medium

  • Particle Shape

    Spherical

  • Particle Size

    2.5 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    100 Å

  • QC Tested

    Peptide

  • Format

    Premier Column

  • Surface Area

    230

  • System

    UHPLC, UPLC

  • Particle Technology

    HSS

  • Technique

    LC

  • USP Classification

    L1

  • Inner Diameter

    2.1 mm

  • Length

    100 mm

  • Carbon Load

    11 %

  • eCord

    Yes

  • UNSPSC

    41115709

  • Application

    Peptide

  • Brand

    XSelect

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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XSelect Premier Peptide HSS T3 Column, 2.5 µm, 2.1 x 100 mm

Designed for separations where silica-based selectivity is desired, the XSelect Premier Peptide HSS T3 Column is a top choice for scientists performing hydrophilic peptide separations or other proteomics-related assays. Thanks to the High-Strength Silica (HSS) particle morphology, this column is equipped to provide superior separation performance resolution when hydrophilic peptide retention is necessary. What’s more, this silica column belongs to the MaxPeak Class of Columns, a designation only given to chromatography consumables that prioritize increased recover for metal-sensitive analytes. MaxPeak technology prevents unwanted analyte-surface interactions from occurring, all leading to a reliable data set that’s easily reproducible. Add the XSelect Premier Peptide HSS T3 Column to your lab’s toolkit today.

For excellent reverse-phase retention of hydrophilic peptides to occur, you need a column that provides superior stability across varying backpressures and temperatures. That’s why the XSelect Premier Peptide HSS T3 Column remains a top choice among scientists working in proteomics. The general-purpose nature of the column makes it a flexible tool that can be used with a wide range of analytes. What’s more, this column is 100% water wettable, which means using a 100% aqueous mobile phase is of no great concern to your workflow. This HPLC column can still provide superior separation performance because of that stability.

Another huge perk of the XSelect Premier Peptide HSS T3 Column is its ability to help you perform method transfer. It doesn’t matter if you need to use this HPLC column to transfer data to UHPLC or UPLC systems, as it prioritizes reproducibility across different LC systems. This all leads to results you can be incredibly confident in. And with improved peak shape and retentivity for basic compounds, this silica column can be used with different analytes without concerns about overall performance.

While the XSelect Premier Peptide HSS T3 Column provides enhanced retention of hydrophilic peptides, we understand that’s not the goal of every scientists. But if you want the separation performance that’s offered by this column, we have good news. Shop XSelect Premier Columns and you’re sure to find the right chromatographic particle and column chemistry to match your assays.

For a lab to run as efficiently as possible, scientists need the right equipment and tools at their disposal. Luckily for you, we have it all at Waters. From quality control reference materials and MS systems to lab informatics, you’ll find exactly what you need to be successful in the lab when you shop with us. Shop for lab equipment now to learn more.

What’s the Max Pressure Allowed By the XSelect Premier Peptide HSS T3 Column?

The XSelect Premier Peptide HSS T3 Column can operate under pressures of up to 18,000 PSI.