Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
90 Å |
Format |
Column |
Surface Area |
200 |
System |
HPLC |
USP Classification |
L1 |
Inner Diameter |
3.9 mm |
Length |
300 mm |
Carbon Load |
10 % |
UNSPSC |
41115709 |
Brand |
Resolve |
Product Type |
Columns |
Units per Package |
1 pk |
What Does Chromatography Sensitivity Refer To?
The signal output per unit concentration or mass of a substance in the mobile phase entering the detector, for example, the slope of a linear calibration curve, is referred to as sensitivity. For concentration-sensitive detectors, sensitivity is sometimes defined as the ratio of peak height to analyte concentration in the peak. In mass-flow-sensitive detectors, it is the ratio of peak height to unit mass. To be a distinct performance attribute, sensitivity must be solely dependent on the chemical measurement procedure, not on scale considerations. Many instrumental and chemical parameters influence the ability to detect (that is, qualify) or measure (that is, quantify) an analyte. In an ideal world, high-efficiency columns elute well-resolved peaks (narrow peak width with good symmetry for maximum peak height). This is in addition to the sensitivity and specificity of the detector. Both separation system interference and electronic component noise should be minimized to ensure optimal sensitivity.