µBondapak C18 Column, 125Å, 10 µm, 3.9 mm X 300 mm, 1/pk
Since it was introduced in 1973 as the first 10-micron particle size bonded reversed-phase column, the µBondapak C18 Columns have been one of the most widely used lab equipment in a range of fields. The unique selectivity of the µBondapak C18 is the result of a controlled combination of hydrophobicity and silanol activity.
The only columns that contain the µBondapak C18 packing material, these offer high efficiency and superior peaks. They also help to control the retention of basic compounds relative to neutral compounds, helping to solve one of the most common challenges with the synthesis of C18 silica packings. No other column provides the same balance between resolution, throughput, and cost.
Waters is committed to supporting columns that provide consistent and reliable column performance to support the use of sorbents and packing materials composed of non-spherical and irregularly shaped particles. These columns have been able to avoid the reduced mechanical stability and lower efficiency that can happen with irregular particle technology.
Offered in multiple particle sizes, µBondapak provides easy transfer of chromatography methodology between particle sizes. This 10 µm can be a starting point for scale-up or to help develop a new purification protocol to help determine the optimal preparative particle size.
Manufactured at Waters' ISO 9002-certified facility with proprietary procedures, µBondapak C18 Guard-Pak Insert, 125Å, 10 µm, 10/pk and columns pass a series of stringent quality control tests to provide superior reproducibility.
FAQs About µBondapak C18 Column, 125Å, 10 µm, 3.9 mm X 300 mm, 1/pk
1. What types of compounds are best analyzed using the µBondapak C18 Column?
The µBondapak C18 Column is ideal for the analysis of hydrophobic and moderately polar compounds due to its reversed-phase design. It is particularly well-suited for small molecules such as pharmaceuticals, environmental contaminants, and organic acids.
This column is also effective for separating neutral and weakly basic analytes, making it a versatile choice for routine laboratory applications. Its high surface area and 125Å pore size allow for consistent retention and sharp peak shapes, making it a reliable tool for compounds requiring robust and reproducible reversed-phase separations.
2. What types of mobile phases are compatible with the µBondapak C18 Column?
The µBondapak C18 Column is compatible with a wide range of mobile phases typically used in reversed-phase chromatography. Common choices include water-organic solvent mixtures such as acetonitrile or methanol, often buffered to control pH. The column is stable across a moderate pH range, allowing for flexibility in method development.
Care should be taken to use mobile phases free of particulates by filtering them prior to use. Proper solvent compatibility ensures consistent performance and extends the column's lifespan, making it an excellent choice for diverse analytical workflows.
3. What is the typical lifespan of the µBondapak C18 Column, and how can it be extended?
The typical lifespan of the µBondapak C18 Column depends on usage and operating conditions but can be extended with proper care. To maximize its longevity, always use filtered and degassed mobile phases to prevent blockages and particulate contamination. Regularly flush the column with appropriate cleaning solvents to remove buildup and prevent degradation of the stationary phase.
Storing the column in recommended solvents when not in use, avoiding extreme pH ranges, and using a guard column for highly complex samples can also significantly extend its lifespan, ensuring consistent and reliable separations over time.
