Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: WAT045905 Symmetry C18 Column, 100Å, 5 µm, 4.6 mm X 150 mm, 1/pk
Symmetry C18 columns feature monofunctionally bonded C18 ligands on a high purity base-deactived silica. Symmetry columns have the tightest column specifications in the industry giving resulting highly reproducible chromatographic results.
Symmetry C18 Column, 100Å, 5 µm, 4.6 mm X 150 mm, 1/pk
Manufactured with high-purity silica and a tightly controlled manufacturing process, Symmetry C18 columns are made to ensure you are offered columns that exceed standards of HPLC column performance. The column to column and batch to batch reproducibility of Symmetry C18 columns are unmatched by all other silica-based LC column brands. The Symmetry C18 columns are available in the format of columns, guards, and cartridges.
Reproducibility is the number one priority in mind when Symmetry C18 columns are being produced and Waters focuses on maintaining the tightest specifications in the HPLC column industry in order to exceed these standards. Engineered with high-purity raw materials, tightly controlled manufacturing processes, and column packing procedures, Symmetry C18 columns provide scientists with the most superior reproducibility available in HPLC columns.
The goal of the Symmetry C18 columns is to provide a reliable, rugged, and robust method for chromatographers across industries. By choosing columns that meet these goals, scientists are able to increase productivity and facilitate easier method transfer across the globe.
With the narrowest ligand surface coverage specifications available, Symmetry C18 columns allow minimal shifts in resolutions and more reproducible results due to the variation in surface coverage on the amitriptyline/acenaphthene alpha specification of Symmetry C18 columns. These columns enable speed for rapid analysis and purification of thousands of samples per day, with a 50% decrease in run time.
Not only is productivity increased with these specifications, but columns are also able to be used for a longer time, saving money and time without sacrificing efficiency. Additionally, a minimal increase in backpressure and minimal change in retention time also provide advantages. By optimizing the hydrolytic stability of the packing material and mechanical stability of the packed bed, Symmetry C18 columns are able to guarantee this consistent performance for up to 10,000 injections.
What separations is the Symmetry C18 Column typically used for?
The Symmetry C18 Column is a workhorse for many labs, functioning as a general-purpose tool for separating non-polar to moderately polar compounds. This makes it a popular choice for analyzing a wide range of samples across various fields, including pharmaceuticals, environmental samples, food analysis, and natural products. The C18 stationary phase, with its water-repelling surface, is ideal for these applications because it interacts favorably with the target analytes through various mechanisms.
What is the pressure range for the Symmetry C18 Column?
The Symmetry C18 Column is built to withstand a maximum pressure of 600 bar (8700 psi).
What is the expected lifespan of the column?
The lifespan of your Symmetry C18 Column depends on how you treat it. Frequent use, harsh mobile phase conditions (like extreme pH or strong solvents), dirty samples, and improper storage can all shorten its life. However, with proper care and use, the column can last for hundreds to thousands of injections.
What is the recommended flow rate range for this column?
The recommended flow rate for the Symmetry C18 Column typically falls within a range of 0.5 to 5 mL/min. There's no single "best" flow rate, as the optimal choice depends on the specific separation you're aiming for. It's a balancing act between resolution, speed, and peak shape. For complex separations requiring high resolution, a slower flow rate might be preferred. Conversely, for faster analysis times, a higher flow rate can be used. Experimentation is often necessary to find the optimal flow rate for your specific application.
What is HPLC used for?
HPLC stands for high-performance liquid chromatography, a chromatographic technique that is used to split a mixture of compounds. HPLC is applied in fields like analytical chemistry, biochemistry, and industrial in order to identify, quantify, and purify the individual components of each mixture.
For pharmaceutical industries, HPLC is used to test products and detect the raw ingredients used to create them through both quantitative and qualitative analysis. The use of HLPC in these cases is regulated by the US FDA before any products can be sold in the market. HPLC offers benefits not only for synthetic drugs and dformulas, but also herbal medicine.
What is HPLC?
High-performance liquid chromatography (HPLC) is an analytical methodology that excels in the separation, identification, and quantification of constituents within a complex mixture. Renowned as the foremost technique extensively employed across laboratories worldwide, this test serves a myriad of purposes with unparalleled versatility.
