Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
100 Å |
Format |
Column |
Surface Area |
335 |
System |
HPLC |
USP Classification |
L1 |
Inner Diameter |
3.9 mm |
Length |
150 mm |
Carbon Load |
19 % |
UNSPSC |
41115709 |
Brand |
Symmetry |
Product Type |
Columns |
Units per Package |
1 pk |
Symmetry C18 Column, 100Å, 5 µm, 3.9 mm X 150 mm, 1/pk
Designed to support the latest analytical methods for biopharmaceutical and pharmaceutical products, the Symmetry C18 column is made with reproducibility as the top priority. Their superior reproducibility comes from a commitment to maintaining the most stringent specifications in the HPLC column industry and engineering processes that use high purity raw materials, tightly controlled manufacturing and packing procedures in order to provide the best and most reproducible HPLC columns on the market.
With the narrowest ligand surface coverage specifications in the industry, Symmetry C18 columns guarantee reproducible results to every chromatographer. By using such tight specifications, Waters is able to produce minimal shifts in resolution along with reproducibility that is consistent not only batch to batch, but also column to column. Along with the Symmetry Silica C18 Method Validation Kit, 300Å, 5 µm, 3.9 mm X 150 mm, 3/pk, your scientists will produce the most efficient and reproducible results possible.
Symmetry columns’ guarantee of consistent performance allows optimization of the two factors most closely tied to the lifetime of a column, hydrolytic stability of the packing material and mechanical stability of the packed bed. Lasting over 10,000 injections with minimal loss of efficiency, minimal increase in backpressure, and minimal change in retention times, Symmetry C18 columns are efficient both in practice and economically.
Delivering superior peak shape on a pH range of 2-8, the bonding density and minimal residual silanol activity of the packing materials allow you to work at a neutral pH or low pH and to optimize your method without compromise to peak shape. This is all done with a speed that enables rapid analysis and purification of thousands of samples per day- run times can be decreased by 50% without any sacrifice to resolution due to a gradient volume that is scaled proportionally to the column volume.
Symmetry C18 columns offer monofunctionally bonded C18 ligands on a high purity base-deactivated silica and Waters’ proprietary end-fittings, simply adding to the endless selection of quality lab equipment from Waters..
What is a C18 column?
C18 columns are columns designed for HPLC (high performance liquid chromatography) and utilize a C18 substance as the stationary phase. These columns are used primarily in environmental sciences or chemical analysis, as well as the pharmaceutical industry, in order to analyze individual parts of chemical mixtures. C18 stationary phases may differ between HPLC columns. C18 simply means that the molecules used tp contain 18 carbon atoms, but other atoms in the molecule may vary and lead to differences between the substances. They can also come in varying sizes, with or without an end cap, with different particle and pore sizes, and with differing abilities to separate components on the pH scale.
What is HPLC?
High-Performance Liquid Chromatography (HPLC) is an analytical methodology that enables the separation, identification, and quantification of elements within a composite substance. This widely adopted technique stands as the paramount approach in laboratories across the globe, serving diverse objectives with unparalleled proficiency.