Chemistry |
C8 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
100 Å |
Format |
Column |
Surface Area |
335 |
System |
HPLC |
USP Classification |
L7 |
Inner Diameter |
4.6 mm |
Length |
250 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Brand |
Symmetry |
Product Type |
Columns |
Units per Package |
1 pk |
Symmetry C8 Column, 100Å, 5 µm, 4.6 mm X 250 mm, 1/pk
If you are looking for columns that exceed the standards for HPLC column performance, Symmetry Columns are manufactured with this goal in mind, using high purity silica and tightly controlled manufacturing processes. The Waters Symmetry C8 Column provides column to column and batch to batch reproducibility that isn’t available with other silica-based LC column brand, giving you the maximum confidence in your methods. Featuring monofunctionally bonded C8 ligands on a high purity base-deactivated silica, Symmetry columns have extraordinarily tight column specifications and the narrowest ligand surface coverage in the industry.
Embrace high bonding density and minimal residual silanol activity in the packing materials as Symmetry columns deliver superior peak shake across the pH range of 2-8, allowing chromatographers to work at neutral or low pH and optimize methods without compromising peak shape. With the speed necessary for high throughput lead generation, you are able to perform rapid analysis and purification of thousands of samples per day with run times decreased by 50% without sacrificing resolution.
Symmetry columns for HPLC are known for their rugged and reproducible performance, with narrow column specification ranges to minimize variation and instill confidence that your methods can be used through time. With these benefits, you can increase your productivity and facilitate easier method transfer between labs and around the globe knowing that you’re using the best in lab equipment.
Each Symmetry column includes a three-page Certificate of Analysis. This reports both the specifications used, which are the tightest in the industry, and the results of the 22 critical tests each column passes before it is able to be labeled as a Symmetry column.
What Is A Column In HPLC?
Columns are the primary lab equipment used in HPLC (high performance liquid chromatography) as the mechanism for separating sample components. The samples pass through the column with the mobile phase and separate its components as it comes out of the column. The material that fills an HPLC column is known as the stationary phase. Most HPLC columns are filled with silica gel, as its particle size and porosity aids in separation of components and is an inert material that will not react with mobile phases. This allows silica columns to be used to analyze the compounds of varying chemical natures. HPLC columns can be normal phase, reverse phase, ion exchange, or size exclusion.