Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
100 Å |
Format |
Column |
Surface Area |
335 |
System |
HPLC |
USP Classification |
L1 |
Inner Diameter |
4.6 mm |
Length |
250 mm |
Carbon Load |
19 % |
UNSPSC |
41115709 |
Brand |
Symmetry |
Product Type |
Columns |
Units per Package |
1 pk |
Symmetry C18 Column, 100Å, 5 µm, 4.6 mm X 250 mm, 1/pk
For preparative high-performance liquid chromatography (HPLC) to be successful, you need an HPLC column that can help you easily overcome analytical challenges and pave the way toward successful MS adoption. If you require a C18 column that sets the standard for HPLC drug assays, then look no further than the Symmetry C18 Column. This HPLC column is outfitted with features to help you achieve incredible peak shape, selectivity and column-to-column reproducibility. When you"re heavily focused on critical performance characteristics within HPLC, you"ll see that the Symmetry C18 Column was developed and designed to suit that attention to perfection. What"s more, we even offer HPLC column protection options for the Symmetry C18 Column, so you can use this column repeatedly without any major concerns about contamination. Order the Symmetry C18 Column and have it shipped to your lab today, or ask your lab manager to include it with your lab's next order from Waters.
At its core function, the Symmetry C18 Column is a silica-based HPLC column that can help you exceed expectations with a dedication to particle separation speed and quality that you"ll want to take advantage of immediately. Achieving repeatable results over the lifetime of a new drug product is critical, and this C18 column is here to help you achieve exactly that and so much more. Order this reversed-phase HPLC column to discover what your assays have been missing.
Speaking of HPLC columns, are you aware of just how many we have to offer?
We have over 5,800 HPLC column formats, 175+ column configurations and 21 unique column phases to suit your various laboratory needs. Browse our HPLC Columns for sale to discover an entire selection of separation tools all built for specific applications.
Going back to HPLC column protection, are you aware that your separation tools can become fouled by repeated use and result in compromised performance?
That"s where VanGuard Column Protection comes into consideration. VanGuard was developed to extend analytical column lifetimes through the application of a guard column cartridge that removes contaminants from the mobile phase stream. Add the Symmetry C18 VanGuard Cartridge to your cart to equip your Symmetry C18 Column with HPLC column guard technology.
At Waters, it"s truly our passion to provide lab environments across the world with the equipment, tools and resources they need to give scientists the best space for success. Shop lab equipment to explore all of our offerings.
FAQs about Waters Symmetry C18 Column
What is the recommended flow rate for the Waters Symmetry C18 Column?
The recommended flow rate for the Waters Symmetry C18 Column is typically between 0.5-1.0 mL/min.
How should I store the Waters Symmetry C18 Column when not in use?
It is recommended to store the Waters Symmetry C18 Column in a cool, dry place away from direct sunlight to maintain its integrity.
Can the Waters Symmetry C18 Column be used for both analytical and preparative chromatography?
Yes, the Waters Symmetry C18 Column is suitable for both analytical and preparative chromatography applications.
What is the particle size of the packing material in the Waters Symmetry C18 Column?
The particle size of the packing material in the Waters Symmetry C18 Column is 5 µm.
How often should the Waters Symmetry C18 Column be replaced?
The lifespan of the Waters Symmetry C18 Column will vary depending on usage, but it is generally recommended to replace the column after 100-200 injections to maintain optimal performance.
What is reproducibility in HPLC?
Reproducibility is a way to express the precision between measurements obtained through different laboratories. This measures the ability of an experiment or calculation to be duplicated by other researchers. In chromatography, it is important that columns are able to create the same results across laboratories to help verify that results are accurate. HPLC columns are created specifically to ensure no slight differences in batches or products may cause issues between experiments.
Is reproducibility different than repeatability?
Reproducibility focuses on whether an entire experiment or study can be reproduced end to end, while repeatability measures variation in measurements taken by single instruments or people under identical conditions.