Chemistry |
Amino (NH2) |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Particle Shape |
Spherical |
Particle Size |
10 µm |
Endfitting Type |
Waters |
Pore Size |
125 Å |
Format |
Column |
System |
HPLC |
USP Classification |
L8 |
Inner Diameter |
3.9 mm |
Length |
300 mm |
UNSPSC |
41115709 |
Application |
Carbohydrate |
Product Type |
Columns |
Units per Package |
1 pk |
Amino (NH2) Column, 125Å, 10 µm, 3.9 mm X 300 mm, 1/pk
The Amino (NH2) Column offers a covalently bonded amino packing ideally suited for the separation of low molecular weight sugars like mono-, di-, and trisaccharides as a Carbohydrate Analysis Column.
The column typically uses a mobile phase consisting of 65 -85% acetonitrile in water. It features a 10 µm silica-based particle designed for HPLC separations. Operating ranges include a 2 – 8 pH range, maximum pressure of 6,000 psi (415 bar), and temperature from ambient to 35 degrees C. Columns are sold 1 to a package, with an inner diameter of 2.9 mm and a length of 300 mm. With a pore size of 125Å, the Amino (NH2) Column is diverse and versatile in its durability and use.
Like all Waters lab equipment, Amino (NH2) Columns are made according to regulatory requirements for column packing and manufacturing. Tests can be performed for benchmarking using Reversed-Phase QC Reference Material.
What Are Examples Of Low Molecular Weight Sugars?
Many foods are made up of abundant and diverse carbohydrates, such as cereal, fruits, potatoes, flour, and vegetables. Carbohydrates are composed of carbon, hydrogen, and oxygen, and can exist as individual molecules or physically or chemically attached to other molecules. A monosaccharide is most often known as a simple, or low molecular weight, sugar. These serve as the building blocks for more complex forms of carbohydrates. This is the simplest form of carbohydrate. Examples include glucose, fructose, galactose, RNA, and DNA.