Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
No |
Silanol Activity |
High |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Pore Size |
90 Å |
Format |
Radial-Pak Column |
Surface Area |
200 |
System |
HPLC |
USP Classification |
L1 |
Inner Diameter |
8 mm |
Length |
100 mm |
Carbon Load |
10 % |
UNSPSC |
41115709 |
Brand |
Resolve |
Product Type |
Columns |
Units per Package |
1 pk |
Resolve C18 Radial-Pak Column, 90Å, 5 µm, 8 mm X 100 mm, 1/pk
The non-end-capped Resolve C18 Radial-Pak Columns are designed with significantly different selectivity compared to similar HPLC packings of those of the Nova-Pak and µBondapak Columns. The change in chromatographic behavior is most commonly noticed with polar compounds, which are typically more retained. The Resolve C18 Radial-Pak Column and other Resolve C18 Column series complement Nova-Pak and µBondapak chemistries. In general, basic compounds can be chromatographed using mobile phase modifiers such as ion-pairing reagents, which reduce excessive tailing. The Resolve C18 Radial-Pak Column, similar to Silica Columns, is available for applications that require higher resolution than what is achievable using irregularly shaped chromatographic particles. The non-end-capped silica surface offers a balance of retention for the separation of polar compounds.
If you want to review the additional lab equipment offered by Waters, please browse through our brochure or visit our website, from where you can shop for lab equipment and ensure you receive genuine and authentic products.
You may also be interested in the Reversed-Phase QC Reference Material; the Quality Control Reference Materials portfolio is a unique collection of standards and mixtures. These products allow the user to evaluate and benchmark their chromatography system before the analysis of critical material. The Reversed-Phase QC Reference Material contains 7 compounds, including uracil, butylparaben, naphthalene, propranolol, dipropyl-phthalate, acenaphthene, and amitriptyline at pH 7. This standard mix should be used to confirm the benchmark performance of your analytical HPLC or UPLC system. This particular QC Reference Material is a specially formulated mix that includes a void marker, neutral, acidic, and basic compounds.
What Is The Average Time Spent To Change A Column?
Column changes are a feasible method development tool in HPLC, and columns can be switched at any time as needed. You can attach the new column with a slow flow of solvent from the injector outlet line to fill the inlet fitting of your column so that you do not leave air in the column entrance. We recommend you figure on flushing a column with at least six columns of a new solvent to re-equilibrate the column before injecting a sample. Plan on ignoring the first injection as typically, the second, third, and subsequent injections will be reproducible.
What Pressure Can Be Used With The Resolve C18 Radial-Pak Column?
With a particle size of 5 µm and pore size of 90 Å, the Resolve C18 Radial-Pak Column is designed to withstand a pressure of 6000 psi (or 415 Bar) along with a pH range of 2 to 8. However, it is always recommended not to cross the maximum range to ensure the best possible column lifetime and effectiveness.