Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: WAT085711 Resolve C18 Column, 90Å, 5 µm, 3.9 mm X 150 mm, 1/pk
The non-encapped Resolve C18 column offers significantly different selectivity compared to similar HPLC packings like Nova-Pak and µBondapak. The change in chromatographic behavior is most commonly noticed with polar compounds, which are typically more retained.
Resolve C18 Column, 90Å, 5 µm, 3.9 mm X 150 mm, 1/pk
For applications that require higher resolution, the non-end-capped Resolve packings offer significantly different chromatographic behavior to Nova-Pak C18 Columns and Bondpak Columns offered by Waters. These behavior changes are most commonly noticed with polar compounds that are typically more retained. Using the Resolve C18 Column, basic compounds can be chromatographed using mobile-phase modifiers, like ion-pairing reagents which reduce excessive tailing.
Waters’ high performance liquid chromatography (HPLC) columns are the dominant lab equipment analytical separations in pharmaceutical, chemical, environmental, and food industries, along with therapeutic drug monitoring applications. With a 5 µm particle size, Waters HPLC columns like the Resolve C18 Column can help increase your productivity while offering higher recoveries and extended column lifetimes. Achieve fast and efficient lab-scale separations for greater throughput while directly scaling between UPLC and HPLC screening.
The non-end-capped Resolve packing materials help to improve reproducibility across columns, make column lifetimes more predictable, prevent lost samples and repeat purification runs, and avoid poor scalability between small and large volume columns. This product works well alongside the Reversed-Phase QC Reference Material from Waters.
All Waters HPLC columns are manufactured under strict quality and regulatory guidelines in order to ensure reproducible performance. Manufacturing occurs in cGMP, ISO 9001:2000 certified plants and each batch is tested chromatographically, then held to narrow specification ranges. Columns arrive with a Performance Test Chromatogram.
What Are Polar Compounds?
Polar compounds are chemical compounds held together by polar covalent bonds. They can also be defined as a chemical species consisting of two or more atoms held together by covalent bonds which are polar in nature, due to the unequal sharing of electrons.
An example of a polar compound is water, scientifically known as H20. Because the covalent bonds between hydrogen and oxygen in the water molecule are polar in nature, it meets the criteria described above. The bond polarity is a result of the electronegativities of hydrogen and oxygen. Other examples include ethanol and hydrogen fluoride.
Where Should I Use Non-End-Capped Columns?
While most applications benefit from end-capping, there are a number of reasons a scientist may prefer non-end-capped options like the Resolve C18 Column. Some legacy applications used non-end-capped materials and need to be reproduced in the same way. Other examples may include the separation of tartaric from quinic acid in fruit juice- few end-capped columns can separate to baseline isocratically, so a non-end-capped option is preferable.
