Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: WAT106005 Symmetry C18 Column, 100Å, 3.5 µm, 2.1 mm X 150 mm, 1/pk
Symmetry C18 Column | 100Å | 3.5 µm | 2.1 mm X 150 mm | 1/pk | WAT106005
Symmetry C18 columns feature monofunctionally bonded C18 ligands on a high purity base-deactived silica. Symmetry columns have the tightest column specifications in the industry giving resulting highly reproducible chromatographic results.
What Is The Process For Column Equilibration For The Symmetry C18 Column?
Before column equilibration can be performed, it is important to ensure mobile phase compatibility before changing to another mobile phase system. Equilibrate the column with a minimum of 10 column volumes of the mobile phase to be used. To avoid precipitating out mobile phase buffers on your column or in the system, flush the column with five column volumes of a water/organic solvent mixture, using the same or lower solvent content as in the desired buffered mobile phase. Please note that 100-200 column volumes may be required for complete equilibration if mobile phase additives are present in low concentrations. Additionally, format containing mobile phases may also require longer initial column equilibration times.
