Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
3.5 µm |
Endfitting Type |
Waters |
Pore Size |
100 Å |
Format |
Column |
Surface Area |
335 |
System |
HPLC |
USP Classification |
L1 |
Inner Diameter |
4.6 mm |
Length |
150 mm |
Carbon Load |
19 % |
UNSPSC |
41115709 |
Brand |
Symmetry |
Product Type |
Columns |
Units per Package |
1 pk |
Symmetry C18 Column, 100Å, 3.5 µm, 4.6 mm X 150 mm, 1/pk
With the tightest specifications in the market, the Symmetry brand of HPLC columns provides the most consistent results available, achieving outstanding year-to-year reproducibility to give long life to your established analytical methods. This reproducibility is the top priority in supplying Symmetry columns, which is why they are always engineered with high purity raw materials and tightly controlled manufacturing processes and column packing procedures. Symmetry C18 Columns offer HPLC columns that can be relied on for rugged and robust methods that enable you to increase productivity and facilitate easier method transfer.
Scientists looking for state of the art, reversed-phase HPLC columns and lab equipment can trust Waters family of products for performance and innovation that leads the industry. The excellent reproducibility of Symmetry columns proves Waters’ commitment to maintaining the tightest specifications in the HPLC column industry.
Symmetry columns can last over 10,000 injections with a minimal loss in efficiency, minimal increase in backpressure, and minimal change in retention time, saving you money as you need to replace columns less often. These columns guarantee consistent performance by optimizing the two major factors in that control column lifetime- hydrolytic stability of the packing material and mechanical stability of the packed bed. Increase column lifetime by also using the Symmetry C18 VanGuard Cartridge, 100Å, 3.5 µm, 3.9 mm X 5 mm, 3/pk alongside your Symmetry C18 Columns.
Delivering a superior peak shape across the pH range of 2-8, Symmetry columns have high bonding density and minimal residual silanol activity of the packing materials. Chromatographers can now work at neutral pH or low pH to optimize methods without compromising peak shape. Symmetry columns are also able to achieve increased resolution with smaller particle size material within the same gradient time, while reducing analysis time by 50% due to shorter column length. You can also maintain resolution by scaling gradient volume proportionally to column volume while increasing throughput.
What Are The Applications Of HPLC?
HPLC, or high performance liquid chromatography, is a chromatographic method used to separate a mixture of compounds in analytical chemistry or biochemistry. The main purpose of the technique is to identify, quantify, and purify a particular analyte or compound. HPLC has a large number of applications, including water purification, detection of impurities in pharmaceutical industries, and liquid-exchange chromatography. It can also be used in ion-exchange chromatography of proteins and pre-concentration of trace components. HPLC is one of the main methods used in the pharmaceutical industry because of the precision of the results, which can be used to analyze finished drug products and their ingredients both quantitatively and qualitatively.