These reversed-phase columns contain Waters innovative embedded polar group technology that “shields” the silica’ residual silanol surface from highly basic analytes. The reduced silanol activity for the SymmetryShield RP8 column significantly improves peak shape and resolution.
Chemistry |
C8 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Bonding Technology |
Shield RP8 |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
100 Å |
Format |
Column |
Surface Area |
335 |
System |
HPLC |
USP Classification |
L7 |
Inner Diameter |
4.6 mm |
Length |
250 mm |
Carbon Load |
15 % |
UNSPSC |
41115709 |
Brand |
Symmetry |
Product Type |
Columns |
Units per Package |
1 pk |
Symmetry Shield RP8 Column, 100Å, 5 µm, 4.6 mm X 250 mm, 1/pk
Setting the standard for performance of peak shape in basic compounds, Symmetry Shield RP8 Columns are reversed-phase columns based on Waters’ patented embedded polar group technology. The innovative technology literally “shields” the silica’s residual surface silanols from highly basic analytes. The embedded polar group sits in close proximity to the silica surface, allowing for further reduction of surface silanols in the Symmetry Shield packing materials.
As a result of this innovative technology, Symmetry Shield RP8 columns are able to deliver a significantly improved peak shape and resolution over a broad pH range. The columns are also able to exhibit less retention of basic compounds than conventional C18 and C8 columns. Symmetry Shield columns deliver USP Tailing Factors of less than 1.2 across a pH range of 2 to 8. With low levels of detection and quantitation, Symmetry Shield columns enable faster new method development, improving productivity in your laboratory. Substituting classic C8 columns with Symmetry Shield RP8 Columns allows for a better separation without changing mobile phase conditions.
For applications that require low organic, highly aqueous mobile phase conditions, chromatographers choose lab equipment with Shield Technology. As reversed-phase, water-wettable particles allow better interactions between the particles and your samples, resulting in stable retention in highly aqueous mobile phases with superior peak shape.
Each Symmetry Column comes with a Certificate of Analysis specific to the batch of packing material contained, including chromatographic results and conditions. A Performance Test Chromatogram is also included, providing individual column test results. In order to maintain excellent reproducibility, Symmetry Shield columns are manufactured using the tightest standards and specifications in the HPLC column industry. Further testing can be performed using the Reversed-Phase QC Reference Material.
How And When Should I Clean Symmetry Shield RP8 Columns?
Contamination of the column may be present if you notice changes in peak shape, peak splitting, shoulders on the peak, shifts in retention, changes in resolutions, or increases in backpressure. This can usually be solved by flushing with a neat organic solvent, taking care not to precipitate buffers. If this flushing procedure does not solve the issue, a cleaning routine that matches the properties of your sample and/or potential contaminant should be used. If column performance remains poor after regeneration, contact a local Waters office for additional support.