Oasis HLB 6 cc Vac Cartridge, 500 mg Sorbent per Cartridge, 60 µm, 30/pk

Designed for the extraction of varying acidic, basic, and neutral compounds of various matrices, the Oasis HLB 6 cc Vac Cartridge uses a simple, generic protocol. These cartridges contain the Oasis HLB sorbent, a universal polymeric reversed-phase sorbent that enables a variety of uses. This reversed-phase sorbent is made from a specific ratio of two monomers to provide superior capacity with a neutral polar hook. Because it is water wettable, this sorbent maintains the capability for higher retention and good recoveries, even in cases where the sorbent may run dry. This prevents you from needing to take extra steps in order to keep sorbent beds from drying out during crucial steps before sample loading. This will save your lab precious sample processing time as well as avoid costly solvent purchase and disposal procedures.

Designed to improve and simplify the process of sample preparation, Oasis HLB Sorbent offers high capacity with low elution volumes (about 1 mL per 60 mg). Compared to silica-based sorbents, there is a higher recovery for polar metabolites like hydroxyatrazine found. Detection limits are always 100 ng/L or better and there are no stopcocks needed for routine analysis when using this sorbent.

Oasis HLB sorbent also increases speed, both within the device and in your laboratory"s workflow. The device"s flow times are not only faster, but also use less vacuum and positive pressure than required in other SPE devices.

As a syringe-barrel cartridge, these Oasis HLB cartridges feature extraordinarily clean syringe barrels and frits and are designed for use with automated SPE instruments and vacuum manifolds. Oasis HLB Sorbent undergoes a proprietary Waters cleaning process with no significant cartridge interferences. This product contains 500 mg of Oasis HLB sorbent per cartridge with 60 µm and comes with 30 in each package. A particle size of 60 µm is best used for more viscous samples.

Shop for lab equipment and explore other unique and relevant products by Waters, such as the PFC Analysis Kit.

Oasis HLB 6 cc Vac Cartridge, 500 mg Sorbent per Cartridge, 60 µm, 30/pk FAQs

What solvents and buffers are compatible with the Oasis HLB sorbent?
The Oasis HLB 6 cc Vac Cartridge is designed for solid-phase extraction (SPE) and is compatible with a wide range of solvents and buffers. The HLB sorbent (Hydrophilic-Lipophilic Balance) is a polymeric material that can retain both polar and nonpolar analytes, making it more versatile than traditional silica-based sorbents.

For conditioning and equilibration, organic solvents such as methanol, acetonitrile, and isopropanol are commonly used, followed by an aqueous buffer or water to prepare the sorbent for sample loading. During sample loading and washing, buffered solutions, water, or low concentrations of organic solvents are often used to remove unwanted matrix components. Elution is typically performed with strong organic solvents, such as methanol, acetonitrile, or a mixture of organic solvent and water with acid or base modifiers (e.g., formic acid, trifluoroacetic acid, or ammonium hydroxide).

What are the best practices for loading, washing, and eluting samples?
To ensure optimal solid-phase extraction (SPE) performance with the Oasis HLB 6 cc Vac Cartridge, follow these key steps:

Condition – Activate the sorbent with an organic solvent (e.g., methanol) followed by water or buffer.

Load – Apply the sample at a controlled flow rate to maximize interaction with the sorbent.

Wash – Use an aqueous buffer or weak organic solvent (e.g., 5-10% methanol) to remove unwanted matrix components.

Elute – Use a strong organic solvent (e.g., methanol or acetonitrile) with pH modifiers to efficiently recover analytes.

Process – Evaporate or reconstitute eluate before LC-MS or GC-MS analysis.

Following these steps ensures high analyte recovery, minimal contamination, and reproducible results.