Where Do GlycoWorks HILIC 1 cc Cartridges Go For Into Sample Preparation Protocol?

In an example protocol for sample preparation from Glycoprotein to enrich FLR labeled glycans using reductive amination reactions, the GlycoWorks HILIC 1 cc Cartridge can be used in two spots. After the denaturation and deglycosylation phase, GlycoWorks HILIC can be used for the extraction of released glycans. Then again after the FLR labeling reaction of glycans, the excess labeling reagent removal also uses the cartridge. The protocol is then finished out by FLR labeling of the glycans appropriately. Always follow included guidelines for specific extraction, purification, and concentration of N-linked glycans validated using IgG.