Sep-Pak C18 Plus Short Cartridge, 360 mg Sorbent per Cartridge, 55 - 105 µm, 50/pk

Confidently use the Sep-Pak sample extraction products for rapid sample preparation. The Waters Sep-Pak C18 Plus Short Cartridge sample extraction products have been trusted by separation scientists throughout the world since 1978. The Sep-Pak cartridges have the ability to solve important sample preparation problems, making them indispensable lab equipment.

Featuring a state-of-the-art packing process as well as a precision design, Sep-Pak C18 Plus Short Cartridges are well suited to robotic applications and make manual use even more convenient. Applications of the Plus cartridges include drugs and their metabolites in serum, plasma, or urine, desalting of peptides, trace organics in environmental water samples, and organic acids in beverages.

Similar in behavior to reversed-phase HPLC columns, the Waters Sep-Pak Plus cartridges have silica-based bonded phase with strong hydrophobicity and can be used to adsorb analytes of even weak hydrophobicity from aqueous solutions.

Successfully reverse flow direction, which is a useful feature for trace enrichment applications, with Sep-Pak Plus cartridges that are suitable for use with liquid and gas samples. The Sep-Pak C18 Plus Short Cartridges are ideal lab equipment as they offer lowered costs (due to less solvent and reagent consumption), increased recoveries (as minimum sample transfer is required), enhanced safety (by ease of disposal and less exposure to toxic agents), improved accuracy (no cross-contamination), and elimination of emulsion problems (less sample handling and complications).

Shop for lab equipment and select the equipment that best suits you and your laboratory needs from a wide range of superior Waters products. The Sep-Pak C18 Plus Short Cartridge, 360 mg Sorbent per Cartridge, 55-105 µm, 2000/pk are also available in a variety of 2000 units per pack for more exhaustive needs and more thorough results.

FAQs About Sep-Pak C18 Plus Short Cartridge, 360 mg Sorbent per Cartridge, 55 - 105 µm, 50/pk

1. What solvents are compatible with the Sep-Pak C18 Cartridge for extraction and elution?
The Sep-Pak C18 Plus Short Cartridge is compatible with a wide range of solvents, making it highly versatile for various extraction and elution protocols. Typical solvents used include methanol, acetonitrile, and isopropanol for conditioning and elution, as well as water or buffer solutions for equilibration and sample loading.
The cartridge also tolerates solvent mixtures and modifiers like formic acid or ammonium hydroxide, depending on the analyte and application. Avoid using highly reactive or incompatible solvents that might damage the sorbent.

2. How should the cartridge be cleaned or regenerated for multiple uses?
To clean or regenerate the Sep-Pak C18 Plus Short Cartridge, flush it with a strong organic solvent such as methanol or acetonitrile to remove retained analytes and contaminants. Follow this with a wash using water or an aqueous buffer to remove polar residues. If acidic or basic conditions were used during sample preparation, ensure thorough neutralization with appropriate buffer solutions.
While the cartridge can be reused for some non-critical applications, its performance may decline with repeated use, particularly for complex or highly contaminated samples. For critical analyses, single-use is recommended to maintain high reproducibility.

3. Can this cartridge be used for both polar and non-polar analytes?
The Sep-Pak C18 Plus Short Cartridge is primarily designed for retaining non-polar and moderately polar compounds due to its hydrophobic C18 (octadecyl) sorbent. It can retain hydrophobic analytes effectively while allowing polar matrix components to pass through during the loading phase. However, with appropriate modifications to the mobile phase or sample conditions, the cartridge can also be used for moderately polar compounds.
For highly polar analytes, alternative sorbents such as hydrophilic interaction liquid chromatography (HILIC) cartridges may be more suitable. Its versatility makes it a valuable tool for a wide range of sample types and applications.