Sep-Pak Silica Plus Long Cartridge, 690 mg Sorbent per Cartridge, 55-105 µm, 50/pk

Use the Sep-Pak Silica Plus Long Cartridge to successfully reverse the flow direction, which is a useful feature for trace enrichment applications. The Sep-Pak Plus cartridges are suitable for use with both liquid and gas samples. The Sep-Pak Silica Plus Long Cartridge offers some key features that are crucial for SPE products. These include lowered costs (due to less solvent and reagent consumption), increased recoveries (as minimum sample transfer is required), enhanced safety (by ease of disposal and less exposure to toxic agents), improved accuracy (no cross-contamination), and elimination of emulsion problems (less sample handling and complications).

The female inlet of the Sep-Pak Silica Plus Long Cartridge accepts male Luer tip which makes it possible for the cartridges to be stacked for increased capacity as well as for multidimensional separations with different column chemistries.

Depend on the Sep-Pak Silica Plus Long Cartridge to deliver rapid sample preparation. The Waters sample extraction products have been trusted by separation scientists throughout the world since 1978. With the ability to solve important sample preparation problems, the Sep-Pak Silica Plus Long Cartridge is indispensable lab equipment.

The Sep-Pak Silica Plus Long Cartridge is well suited to robotic applications and makes manual use even more convenient. This is because it features a state-of-the-art packing process as well as a precision design. The cartridge can be used across a wide range of applications including drugs and their metabolites in serum, plasma, or urine, desalting of peptides, trace organics in environmental water samples, and organic acids in beverages.

The Waters Sep-Pak Silica Plus Long Cartridges have a silica-based bonded phase with strong hydrophobicity and can be used to adsorb analytes of even weak hydrophobicity from aqueous solutions.

Use our website to find the different formats of the cartridges in our portfolio. You can also shop for lab equipment, and check all related products.

You may also be interested in TruView LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk which makes it easy to securely store your analytes.

Sep-Pak Silica Plus Long Cartridge, 690 mg Sorbent per Cartridge, 55 - 105 µm, 50/pk FAQs

How does silica-based SPE differ from polymer-based SPE?
Silica-based solid-phase extraction (SPE) differs from polymer-based SPE primarily in its selectivity, retention mechanism, and chemical stability. Silica sorbents, such as those in the Sep-Pak Silica Plus Long Cartridge, operate via adsorption chromatography, meaning analytes interact with the polar silica surface through hydrogen bonding, dipole interactions, and van der Waals forces. This makes silica particularly effective for polar compound retention and normal-phase SPE applications.

In contrast, polymeric sorbents (such as Oasis HLB) rely on hydrophobic and hydrophilic interactions, offering broader pH stability (0-14) and greater resistance to solvent degradation. While silica sorbents typically degrade under extreme pH conditions, they provide sharper separations for highly polar analytes and are preferred for normal-phase extractions where water is minimized.

What is the particle size range (55-105 µm) and how does it affect separations?
The 55-105 µm particle size of the Sep-Pak Silica Plus Long Cartridge affects separation efficiency, flow rates, and analyte retention. Smaller particles typically offer higher resolution due to increased surface area and tighter packing, improving interaction between analytes and the silica sorbent. However, smaller particles create higher backpressure, which may slow down processing in vacuum or positive-pressure SPE systems.

The 55-105 µm range strikes a balance between efficiency and ease of use, allowing for moderate flow rates without excessive pressure buildup. This particle size is well-suited for normal-phase SPE applications, providing consistent retention of polar compounds while ensuring efficient sample processing. The range also supports scalability, making it applicable to routine and high-throughput sample extractions.

Can the Sep-Pak Silica Plus Cartridge be reused?
The Sep-Pak Silica Plus Long Cartridge is generally designed for single-use applications to ensure consistent and contamination-free extractions. Since silica-based sorbents operate via adsorption chromatography, they can irreversibly retain some analytes, leading to carryover and reduced extraction efficiency if reused.