Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: WAT023635 Sep-Pak C18 Plus Long Cartridge, 820 mg Sorbent per Cartridge, 55 - 105 µm, 50/pk
Silica-based bonded phase with strong hydrophobicity; used to adsorb analytes of even weak hydrophobicity from aqueous solutions; typical applications include drugs and their metabolites in serum, plasma or urine, desalting of peptides, trace organics in environmental water samples, organic acids in beverages; similar in behavior to reversed-phase HPLC columns.
Sep-Pak C18 Plus Long Cartridge, 820 mg Sorbent per Cartridge, 55-105 µm, 50/pk
Recognized throughout the world, Sep-Pak Solid Phase Extraction (SPE) products are the most referenced family of SPE products for superior sample preparation. The Sep-Pak C18 Plus Long Cartridge is one piece of this family, offering behavior similar to reversed-phase HPLC columns and its own unique benefits. With a silica-based bonded phase that has strong hydrophobicity, this piece of lab equipment can be used to adsorb analytes of even weak hydrophobicity from aqueous solutions.
Sep-Pak C18 Plus Long Cartridges are often chosen for laboratories specializing in applications including drugs and their metabolites in serum, plasma, or urine, as well as the desalting of peptides, trace organics in environmental water samples, and organic acids in beverages. The cartridge is designed to allow easy integration into existing sample preparation protocol and reduce background interference. This reduces solvent waste by eliminating unnecessary solvent pre-washing steps, which are often required for trace residue methods.
The silica-based sorbent included in each Sep-Pak C18 Plus Long Cartridge is an unbonded, highly activated silica stationary phase suited to elution with more polar solvents like polar esters, ethers, alcohols, acetonitrile, or water. The silica provides a slightly acidic surface for moderate cation-exchange interactions in aqueous samples.
Each order contains 50 columns, each of which contains 820 mg of sorbent. All Sep-Pak materials are manufactured in a Waters ISO 13485 and ISO 9002 registered facility that is in compliance with cGMP guidelines for the United States Food and Drug Administration for the production of Class 1 Medical Devices. Columns are compatible with other Sep-Pak equipment, such as the Long Needle Valve for Sep-Pak Device Vacuum Manifold, 20/pk.
What Are the Typical Steps In Solid Phase Extraction?
While not all steps are needed for each separation, Sep-Pak cartridges are part of SPE, which follows a general process. This process begins with preparation of the sample, during which it is applied to the cartridge. The cartridge is then equilibrated, and if it is a reversed-phase sorbent, a conditioning step is required as well.
From there, the sample can be loaded onto the cartridge.
The wash step is then performed, removing interferences while retaining the analyte. The final step in the process is elution using an appropriate solvent.
