Sep-Pak C18 Classic Cartridge, 360 mg Sorbent per Cartridge, 55 - 105 µm, 50/pk

Use the Sep-Pak C18 Classic Cartridge to extract your solvents, solutions, and samples with ease and conveniently transfer them, without worrying about spillage or sample loss due to evaporation. The Sep-Pak C18 Classic Cartridge is the ideal lab equipment as it is a precision chromatographic tool for sample enrichment and purification.

The Sep-Pak C18 Classic Cartridge contains the highest quality packings that have been designed and made specifically for sample preparation. The packings are clean, dry, reproducible in activity and capacity. The packing of the Sep-Pak C18 Classic Cartridge also offers optimal surface area, pore-size, particle-size distributions.

You can use the Sep-Pak C18 Classic Cartridge with the certainty that its product integrity is maintained. This is because the lab equipment is sealed in a specially designed, non-contaminating poly-foil pouch that maintains product integrity, packing inactivity, and purity until the package is opened.

The Sep-Pak C18 Classic Cartridge utilizes a blend of HD and UHMW polyethylenes for the 20 µm frit in the design. The frit gives excellent solvent resistance, very low extractable levels, and good flow properties. It also performs the function of a depth filter that retains small amounts of sample debris.

You may also be interested in the Long Needle Valve for Sep-Pak Device Vacuum Manifold, 20/pk. The vacuum manifold makes it possible for the processing of multiple samples at the same time. The manifold is easy to use and allows steady extraction and filtration.

To check all related products and other variants of the same listing, shop for lab equipment, as well as to see our complete range of products and latest offerings, refer to our product selection. Our catalog also contains information regarding our field offices and how to contact our members of staff for inquiries.

Sep-Pak C18 Classic Cartridge, 360 mg Sorbent per Cartridge, 55 - 105 µm, 50/pk FAQs

1. How much sample can the Sep-Pak C18 Classic Cartridge typically handle?
The Sep-Pak C18 Classic Cartridge, with a sorbent mass of 360 mg, is designed to handle moderate to large sample volumes depending on the specific application and analyte concentration. For typical extractions, the cartridge can process samples up to several milliliters in volume, though optimal performance is often achieved with sample loads up to around 5 mL.
This capacity makes the Classic Cartridge ideal for extractions in applications like environmental monitoring, food testing, and pharmaceutical analysis, where larger sample volumes are often required to achieve detectable analyte levels. For more accurate results, sample volumes may need adjustment based on analyte solubility and concentration.

2. Is the Sep-Pak C18 Classic Cartridge recommended for peptide or protein purification?
Yes, the Sep-Pak C18 Classic Cartridge can be used for peptide and protein purification, especially when dealing with hydrophobic or partially hydrophobic biomolecules. The C18 phase’s affinity for nonpolar compounds makes it suitable for binding and eluting peptides and smaller proteins. However, for proteins with high molecular weights, the C18 sorbent may have limited binding capacity due to steric constraints, so a sample-specific evaluation is recommended.
To achieve the best results, optimize binding and elution steps with buffers adjusted for pH and ionic strength to maximize protein retention without denaturation. Applications involving small peptides and moderately hydrophobic proteins typically yield strong results.

3. How do I prepare the Sep-Pak C18 Classic Cartridge for use?
To prepare the Sep-Pak C18 Classic Cartridge for use, start by conditioning it with a compatible solvent to activate the C18 sorbent phase and improve analyte retention. Begin with a wetting solvent, such as methanol or acetonitrile, followed by a rinsing step with deionized water to eliminate any air bubbles or impurities.
Once conditioned, equilibrate the cartridge with a weak solvent or buffer suited to your specific analyte, often a dilute aqueous solution. This preparation optimizes the surface for interactions with hydrophobic compounds.