Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: WAT106202 Oasis HLB 6 cc Vac Cartridge, 200 mg Sorbent per Cartridge, 30 µm, 30/pk
The 6 cc Oasis HLB cartridges contain the Oasis HLB sorbent, which is a universal polymeric reversed-phase sorbent that was developed for the extraction of a wide range of acidic, basic, and neutral compounds from various matrices using a simple, generic protocol. Since the Oasis HLB sorbent is water wettable, it maintains its capability for higher retention and excellent recoveries even if the sorbent runs dry, which means there is no need to take extraordinary steps to keep the sorbent beds from drying out during the critical steps prior to sample loading. These syringe-barrel-type cartridges are designed for use with vacuum manifolds and automated SPE instruments.
Oasis HLB 6 cc Vac Cartridge, 200 mg Sorbent per Cartridge, 30 µm, 30/pk
When Waters introduced Oasis HLB, the way scientists performed solid-phase extraction (SPE) was forever changed by the innovation. Using a water-wettable copolymer that remains stable from pH 0-14, Oasis HLB opened the door for a range of solid-phase extraction method development possibilities and became the gold standard in reversed-phase SPE around the globe.
Eliminate condition and equilibrium steps from conventional solid-phase extraction protocols, reducing the number of processing steps from 5 to 3, with the innovative development of the Oasis HLB 6 cc Vac Cartridge. Because it is a water-wettable sorbent, Oasis HLB allows analytes to interact with the sorbent and be retained when loaded directly onto the sorbent in an aqueous sample solution. This change allows an average reduction in solvent consumption of up to 70%, with a 40% saving in sample preparation time.
Attain excellent recoveries due to the water-wettable properties, even in cases where the sorbent may run dry. This prevents scientists from having to take extra steps to keep the sorbent beds from drying out during critical steps prior to sample loading.
Enhance retention of polar analytes due to the excellent reversed-phased capacity with a neutral polar hook of the Oasis HLB 6 cc Vac Cartridge. Oasis HLB acts as a universal, reversed-phase sorbent for acidic, neutral, and basic compounds. It is made from a specific ratio of two monomers- the hydrophilic N-vinylpyrrolidone and the lipophilic divinylbenzene. With higher retention capacity, more analytes are retained with less breakthrough, leading to improved recovery and overall reproducibility of your laboratory's SPE method.
Oasis HLB sorbent uses a patented design in order to concentrate a sample up to 15 times without risking evaporation or reconstitution in the process. The sorbent is delivered in syringe-barrel cartridges, designed for use with vacuum manifolds and automated SPE instruments.
FAQs About the Oasis HLB 6 cc Vac Cartridge, 200 mg Sorbent per Cartridge, 30 µm, 30/pk
1.What is the sorbent material and amount in the Oasis HLB 6 cc Vac Cartridge? Each Oasis HLB 6 cc Vac Cartridge contains 200 mg of HLB sorbent. The HLB sorbent is a hydrophilic-lipophilic balanced polymer designed to retain a wide variety of analytes, from highly polar to non-polar compounds. The particle size of the HLB sorbent in the Oasis cartridge is 30 µm. This particle size ensures efficient flow rates and optimal retention of analytes, providing high recovery and reproducibility.
2.What are the optimal operating conditions for this cartridge?
Optimal operating conditions for the Oasis HLB 6 cc Vac Cartridge include using appropriate solvents for conditioning, loading, washing, and eluting the analytes. The cartridge is compatible with various solvents, including water, methanol, acetonitrile, and buffers. It is recommended that specific protocols be followed for each application to achieve the best extraction efficiency and analyte recovery.
3. What are the typical flow rates for using this cartridge?
The typical flow rates for the Oasis HLB 6 cc Vac Cartridge range from 1 to 10 mL per minute, depending on the sample viscosity and the applied vacuum pressure. It is important to optimize the flow rate to ensure efficient analyte retention and elution while avoiding breakthroughs or clogging.
4.What solvents are compatible with the Oasis HLB 6 cc Vac Cartridge?
The Oasis HLB 6 cc Vac Cartridge is compatible with a wide range of solvents, including water, methanol, acetonitrile, and various aqueous buffers. This compatibility allows for flexibility in method development and application across different sample types and extraction protocols.
What is SPE?
SPE, or solid-phase extraction, is a sample preparation technology that is routinely used in analytical laboratories to perform the extraction of analytes from a complex matrix. This process enables the extraction, clean up, and concentration of analytes prior to their quantification.
The basic principle of SPE is the partitioning of compounds between two phases of solid and liquid, where there must be a greater affinity for the solid-phase than the sample matrix.
How is SPE used?
Using SPE, scientists are able to prevent most problems that may occur during a liquid-liquid extraction while improving quantitative recovery yields. The technique is rapid, with most extractions happening in under 30 minutes, is simple to perform, and can be automated in many cases.
