Designed alongside the BioResolve SCX columns, the IonHance CX-MS pH 10X Concentrates A and B, in MS Certified LDPE Containers, were carefully optimized for use in creating a pH 5.0 to 8.5 gradient for LC/MS analysis of mAb charge variant separations.
Separation Mode |
Ion Exchange Chromatography |
Detection Method |
LCMS |
Formulation |
Liquid |
Storage Condition |
Room Temperature |
System Type |
LC/MS |
Shelf Life |
12 Months |
Shelf life upon reconstitution |
1 Week |
Volume |
100 mL |
Type |
Mobile Phase |
UNSPSC |
41116107 |
Application |
Protein |
Brand |
IonHance |
Product Type |
Standard Kits |
Units per Package |
1 pk |
IonHance CX-MS pH Concentrates A & B Kit in MS Certified LDPE Containers (10X Concentrates)
The IonHance CX-MS pH Concentrates A & B Kit has been designed to be utilized alongside the BioResolve SCX columns. The IonHance CX-MS pH 10X Concentrates A and B, in MS Certified LDPE Containers, were carefully optimized for use in creating a pH 5.0 to 8.5 gradient for LC/MS analysis of mAb charge variant separations.
Generally, mobile-phase additives, such as strong acids or buffers, are added into the liquid chromatography setup to improve analyte retentivity and peak shape. For MS applications, high-purity, mobile-phase additives need to be included as a necessary step to reduce background contribution and ensure data quality.
IonHance modifiers are used for LC and LC-MS techniques, such as reversed-phase separations of proteins, peptides, and small molecules. The IonHance modifiers have been carefully designed to assist in achieving low metal content and high-quality standards. These modifiers can assist in improving sensitivity, retentivity, and peak shape for even the toughest separations; hence the IonHance CX-MS pH Concentrates A & B Kit containing the IonHance modifiers are used in conjunction with alongside the BioResolve SCX columns.
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You might also be interested in Reversed-Phase QC Reference Material; The Quality Control Reference Materials portfolio is a unique collection of standards and mixtures. These products allow the user to evaluate and benchmark their chromatography system before the analysis of critical material. The products in the portfolio are all specially formulated based on the expertise of Waters scientists. The Reversed-Phase QC Reference Material is a significantly complex reference material mixture in that it contains compounds that could be challenging to separate.
It is designed to provide this complexity and is appropriate when complex assays are routinely performed. It can be used with a variety of column chemistries, dimensions, and system hardware. The Reversed-Phase QC Reference Material contains 7 compounds, including uracil, butylparaben, naphthalene, propranolol, di-propylphthalate, acenaphthene, and amitriptyline at pH 7. This standard mix should be used to confirm the benchmark performance of your analytical HPLC or UPLC system. This particular QC Reference Material is a specially formulated mix that includes a void marker, neutral, acidic, and basic compounds.
What Impact Does Polarity Have On Separation In Chromatography?
Polar molecules are more strongly attracted to polar solvents and so move further if a polar solvent is used as opposed to a non-polar solvent. The distance that spots move can be compared to the overall distance the solvent has moved and comparisons and measurements made.