RapiGest SF, 50 mg, 1/pk

Originally created as an enzyme-friendly surfactant meant to assist in-solution protein digestion, RapiGest SF is an innovative evolution of the product that helps to improve protein solubility during preparation of samples. As a Waters patented reagent, this lab equipment, RapiGest SF is used to enhance in-gel and in-solution enzymatic digestions of proteins. It is particularly beneficial when preparing protein samples for mass spectrometry (MS) analysis because of the ability to avoid high levels of background noise that results from other common surfactants.

RapiGest SF has also been shown to be useful as a method to solubilize glycoproteins before deglycosylation with enzymes like PNGase F. This process is also beneficial as proteins are made more vulnerable to enzymatic cleavage while avoiding inhibition of enzymatic activity, and remaining heat stable to support high-temperature digestions.

Use of RapiGest SF will not modify peptides or suppress endoprotease activity, making it compatible with a wide range of enzymes, including Trypsin, PNGase F, Lys-C, Arg-C, Asp-N, Glu-C. It can also be easily removed after use to allow for MALDI-TOF MS, LC, or LC/MS analyses of already digested samples.

This surfactant works through mild protein denaturation, which opens protein structure and exposes the proteolytic sites to enzymatic cleavage. These enzymes are left more resistant than common proteins, allowing them to remain active in RapiGest SF solutions. The hydrolysis in acidic conditions that RapiGest SF undergoes allows it to be used to remove surfactant from solutions when necessary.

To improve the speed and completeness of your lab's protein enzymatic digestions and enable high-sequence-coverage peptide mapping of therapeutic proteins, RapiGest SF, 1 mg, 1/pk is a surfactant fit to your needs.

This vial contains 50 mg, intended for large scale digestions. Simplify your preparation protocols and improve throughout of analyses with this innovative solution.