SKU: 186002866
MassPREP Digestion Standard Mix 2

MassPREP Digestion Standard Mix | 186002866


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Product Description

MassPREP™ Protein Digestion Standard Mix 2 consists of a range of molar ratios for tryptically digested proteins; Yeast Enolase (SwissProt P00924), Phosphorylase b (SwissProt P00489), Yeast Alcohol Dehydrogenase (ADH, SwissProt P00330) and Bovine Serum Albumin (BSA, SwissProt P02769). The exact ratio is published on the COA. This standard is used for verification of Waters PLGS software.

Specifications

  • Separation Mode

    Reversed Phase

  • Detection Method

    UV

  • Formulation

    Solid

  • Storage Condition

    Room Temperature

  • System Type

    LC

  • Shelf Life

    60 Months

  • Shelf life upon reconstitution

    1 Week

  • Shipping Condition

    Ambient

  • Type

    Digestion

  • UNSPSC

    41116107

  • Application

    System Performance

  • Brand

    MassPREP

  • Product Type

    Standards

  • Units per Package

    1 pk

Product Support

Documents

Documents



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MassPREP Digestion Standard Mix 2

A variety of molar ratios for frequently digested proteins, including yeast enolase (SwissProt P00924), phosphorylase b (SwissProt P00489), yeast alcohol dehydrogenase (ADH, SwissProt P00330), and bovine serum albumin, are included in the MassPREPTM Protein Digestion Standard Mix 2. (BSA, SwissProt P02769). The COA makes the precise ratio available. The Waters PLGS program is verified using this standard.

The performance of HPLC columns, high-performance liquid chromatography, and mass spectrometry apparatus used to examine this class of compounds can be assessed using the MassPREP Protein Digestion Standards. Waters provides a number of expertly produced, quality-control approved peptide standards that may be used to evaluate the system's performance before using a column and LC system to investigate precious materials. Before employing a column and LC system to examine priceless materials, Waters offers a number of carefully crafted, quality-control approved peptide standards that may be used to assess the system's effectiveness.

This particular MassPREP™ Protein Digestion Standard Mix 2 has a shelf life of up to 60 months or one week after reconstitution. MassPREP Peptide Standard Mixtures should be stored at room temperature.

You can review our catalog or browse through our website to shop for lab equipment as per your laboratory requirements. You may also check out the vast array of offerings and products on our website, which includes additional lab equipment and standards that are compatible with the MassPREP DIgestion Standard listed here.

You may also be interested in MassPREP Enolase Digestion Standard; Trypsin of sequencing grade is used to digest yeast enolase (SwissProt P00924) to create the Waters MassPREP™ Enolase digestion standard. No standard protein, trypsin, or other hydrophilic substances are present in the finished protein digest. Every protein digestion standard batch is made in accordance with stringent quality control standards. The Certificate of Analysis report that is included contains the test results for each batch of the digestion standard. The performance evaluation of HPLC columns, high performance liquid chromatography, and mass spectrometry equipment used for the analysis of this class of substances are just a few of the uses for MassPREP™ Protein Digestion Standards.

What Techniques Are Advisable For Isolating And Preparing Analytes?

To separate and prepare specific sample components, two frequently employed solid-phase extraction (SPE) techniques are utilized. When the concentrations of the target chemicals are too low to achieve precise quantification, the retention-cleanup-elution approach is commonly employed. This method allows for the concentration of diluted samples and trace enrichment of the target chemicals. On the other hand, if the desired sample component is already present at a high concentration, a pass-through cleanup method can be utilized. However, it's important to note that the pass-through cleanup approach does not result in sample enrichment.