AccQ-Tag Ultra Eluent B, 1L

Based on the needs of scientists, Waters designed the AccQ-Tag method to enable laboratories to obtain accurate amino acid composition from protein/peptide hydrolysates, physiologic fluids, feeds, foods, pharmaceutical preparations, and myriad other samples using traditional HPLC or UPLC instrumentation. The 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) reagent is able to react with both primary and secondary amines. These processes do not interfere with the separation, identification, and quantitation of amino acids contained in peptide or protein hydrolysates. The derivatives remain stable for days, allowing batch processing and repeat analysis.

This versatile and proven method is the leading chemistry solution for amino acid analysis, specifically created for accurate, high-sensitivity UPLC analyses. With a range of specific solutions and lab equipment, the AccQ-Tag Method is able to derivatize amino acids, separate the derivatives using reversed-phase HPLC and UPLC, and accurately quantitates the derivatives using external standards and optimal detection.

AccQ-Tag Ultra Eluent B is used in the mobile phase and provided in a 1-liter volume bottle. AccQ-Tag Ultra utilizes AccQ-Fluor Reagent in order to quickly and easily derivatize both primary and secondary amino acids in a single step, generating unparalleled quantitative results. The comprehensive and fully tested reagents, columns, and eluents that make up the AccQ-Tag Ultra Chemistry Kit, including AccQ-Tag Ultra Eluent B.

Compared to AccQ-Tag Ultra Eluent A, Eluent B is suited to a larger number of free amino acids found in process samples, such as cell culture or fermentation broths. When choosing the proper eluent, a number of factors should be considered, including removal of particulates, sample mass for derivatization, whether sample neutralization is required, and use of excess derivatization reagent based on sample mass.