Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
The Hi3 Ecoli and Phos B standards are a specialized set of 6 synthetically prepared and highly purified peptides that provide the top 6 ionizing peptides of both the E.coli ClpB and rabbit Phosphorylase B proteins. These standards are quantitated via AAA to equimolar ratios to provide greater accuracy to each analysis. They are greater than 95% pure and have been verified by mass identification. These standards are primarily intended for use with the Hi3 quantification method for MSE proteomics data processed with ProteinLynx Global Server. The E. coli standard is intended for samples of animal origin, and the Phos B standard is intended for samples of microbial origin. The standards may also be of use in the evaluation and benchmarking of proteomic LC/MS systems comprised of nanoACQUITY UPLC and Synapt and Xevo time-of-flight mass spectrometers.
The Hi3 E. Coli and Phos B standards are a specialized collection of six highly pure synthetic peptides that represent the top six ionizing peptides of both the rabbit Phosphorylase B and E. coli ClpB proteins. For each analysis, these standards are quantified using AAA to equimolar ratios to increase precision. They have been mass-identified and found to be more than 95% pure.
These standards are primarily meant to be used with the ProteinLynx Global Server's MSE proteomics data processing Hi3 quantification method. The Phos B standard is meant for samples of microbial origin, while the E. coli standard is meant for samples of animal origin. The benchmarking and assessment of proteomic LC/MS systems made up of nanoACQUITY UPLC, Synapt, and Xevo time-of-flight mass spectrometers may also benefit from the standards.
Explore our website to uncover more Waters products and collections, as well as to shop for lab equipment based on your lab's requirements. You can also reach out to a member of our global support team through our website. Our customer support agents can assist you if you have any questions or problems. Additionally, they can assist you in selecting the finest options for your chromatographic and laboratory requirements.
We also advise checking out Oasis Method Development 96-well µElution Plate, 2 mg Sorbent per Well, 30 µm, 1/pk; Development of The Oasis Method Elution Plate was created primarily to remove uncertainty from the development of SPE methods. The four Oasis mixed-mode, ion-exchange chemistries—strong and weak anion exchangers (MAX and WAX), as well as strong and weak cation exchangers—are each included in three rows in the proprietary 96-well plate configuration (MCX and WCX). The creation of sample preparation methods can be completed fast and simply using just two SPE techniques. For automated, high throughput SPE, the Oasis Elution Sorbent Selection plate is compatible with the majority of liquid-handling robotic systems.
How Should I Go About Filtering in a Liquid Chromatography Lab Setting?
Before adding a sample to the syringe, draw around 1 mL of air into the device. This will enable the air to exit the syringe with the sample. The filter device's fluid retention is reduced by this "air purge." The filtered solution should now be injected into the syringe. Twist the filled syringe onto the filter device while holding the filter device in one hand without using excessive effort. Start the filtration process by applying light pressure.
