Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
The Cytochrome C Digestion Standard was prepared by digesting Bovine Heart Cytochrome C (Uniprot # P62894) with sequencing grade trypsin. Each batch of digestion standard is prepared under strict quality control criteria confirmed on the included certificate of analysis. Protein digestion standards may be used for a variety of applications, including evaluation and benchmarking of HPLC and UPLC columns or evaluation of mass spectrometry and chromatography instrumentation performance.
By digesting Bovine Heart Cytochrome C using sequencing grade trypsin, the Cytochrome C Digestion Standard was developed. Each batch of digestion standard is made under rigorous quality control guidelines, as evidenced by the certificate of analysis supplied. Protein digestion standards can be used for a variety of purposes, including evaluating and benchmarking HPLC and UPLC columns, as well as assessing the performance of mass spectrometry and chromatography instruments.
1nmol of protein was digested to provide the protein digestion standards. The suggested sample dilution for HPLC column evaluation is 200µl of 0.055 percent trifluoroacetic acid in water. At room temperature, the lyophilized powder remains stable until the expiration date written on the label. It has a 60-month shelf life. When the standard is reconstituted in 0.055 percent trifluoroacetic acid in water and held at 8°C for one week, the solution is stable. The standard's stability can be increased by freezing samples at -20C.
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You may also be interested in Reversed-Phase QC Reference Material; the Quality Control Reference Materials portfolio is a unique collection of standards and mixtures. These products allow the user to evaluate and benchmark their chromatography system before the analysis of critical material. The products in the portfolio are all specially formulated based on the expertise of Waters scientists. The Reversed-Phase QC Reference Material is a significantly complex reference material mixture in that it contains compounds that could be challenging to separate. It is designed to provide this complexity and is appropriate when complex assays are routinely performed. It can be used with a variety of column chemistries, dimensions, and system hardware. The Reversed-Phase QC Reference Material contains 7 compounds, including uracil, butylparaben, naphthalene, propranolol, di-propyl-phthalate, acenaphthene, and amitriptyline at pH 7. This standard mix should be used to confirm the benchmark performance of your analytical HPLC or UPLC system. This particular QC Reference Material is a specially formulated mix that includes a void marker, neutral, acidic, and basic compounds.
What Does The Term Elution Refer?
Elution refers to the chromatographic process of extracting a substance adsorbed onto a solid adsorbent using a solvent, or the detachment of an antibody from its antigen. The eluent or eluant acts as the "carrier" component of the mobile phase, facilitating the transportation of analytes through the chromatograph during the elution process.
