Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
Analysis of intact proteins by MS is a powerful approach for protein identification and assignment of protein modifications. The Intact mAb Mass Check Standard is a LC/MS standard used as a qualitative tool for intact mass analysis. It is an intact mouse protein purified by Protein-A with a known molecular weight, ideal for higher molecular weight intact mass measurements. It is provided in a Waters Max Recovery Vial PN 18600327c. This standard is designed to be used for routine MS optimization, system setup and performance testing.
Add the Intact mAb Mass Check Standard to your lab as the analysis of intact proteins by MS is a powerful approach for protein identification and assignment of protein modifications. The Waters Intact mAb Mass Check Standard is an LC/MS standard used as a qualitative tool for intact mass analysis and is an intact mouse protein purified by Protein-A with a known molecular weight, ideal for higher molecular weight intact mass measurements.
The standard is delivered to you in a Waters Max Recovery Vial, ensuring its quality and purity. This standard is designed to be used for routine MS optimization, system setup, and performance testing.
The Intact mAb Mass Check Standard is a fully characterized monoclonal antibody standard. A monoclonal antibody is a glycosylated protein with a molecular weight of about 150 kDa.
Many different biopharmaceutical organizations perform the routine analytical task of Intact mass analysis of mAb by LC/MS. It provides a rapid method to investigate the integrity of the biological sample. Low molecular weight protein standards that are commonly available for instrument tuning or performance checking (e.g., myoglobin) are not well suited to check the instrument performance when the analysis of monoclonal antibody is needed because analytical (instrument) conditions may greatly deviate from that for mAb analysis due to molecular weight and structure difference (e.g., glycosylation).
The mAb sample is ready for injection/analysis as a true intact mAb reference standard upon a simple reconstitution step in your lab. It serves as a convenient reference sample for MS instrument tuning and calibration check for intact mass analysis of high molecular weight proteins because the mAb sample has a similar MW range to conventional therapeutic monoclonal antibodies.
The recommended solubilization of the Intact mAb Mass Check Standard is in 100-200 µL of DI water. Store at -20 °C immediately upon arrival.
Intact mass analysis is the measurement of a protein's total molecular weight by mass spectrometry (MS) without prior digestion or fragmentation of the molecule of interest. The observed mass can then be compared to the expected mass for a given amino acid sequence.
