Intact mAb Mass Check Standard
Add the Intact mAb Mass Check Standard to your lab as the analysis of intact proteins by MS is a powerful approach for protein identification and assignment of protein modifications. The Waters Intact mAb Mass Check Standard is an LC/MS standard used as a qualitative tool for intact mass analysis and is an intact mouse protein purified by Protein-A with a known molecular weight, ideal for higher molecular weight intact mass measurements.
The standard is delivered to you in a Waters Max Recovery Vial, ensuring its quality and purity. This standard is designed to be used for routine MS optimization, system setup, and performance testing.
The Intact mAb Mass Check Standard is a fully characterized monoclonal antibody standard. A monoclonal antibody is a glycosylated protein with a molecular weight of about 150 kDa.
Many different biopharmaceutical organizations perform the routine analytical task of Intact mass analysis of mAb by LC/MS. It provides a rapid method to investigate the integrity of the biological sample. Low molecular weight protein standards that are commonly available for instrument tuning or performance checking (e.g., myoglobin) are not well suited to check the instrument performance when the analysis of monoclonal antibody is needed because analytical (instrument) conditions may greatly deviate from that for mAb analysis due to molecular weight and structure difference (e.g., glycosylation).
The mAb sample is ready for injection/analysis as a true intact mAb reference standard upon a simple reconstitution step in your lab. It serves as a convenient reference sample for MS instrument tuning and calibration check for intact mass analysis of high molecular weight proteins because the mAb sample has a similar MW range to conventional therapeutic monoclonal antibodies.
The recommended solubilization of the Intact mAb Mass Check Standard is in 100-200 µL of DI water. Store at -20 °C immediately upon arrival.
To shop for lab equipment such as the standard listed here, or to find similar reagents, order our catalog, or browse through our website. You may want to explore the BEH450 SEC Protein Standard Mix as well.
Otto SPEcialist Positive Pressure Manifold
For chromatographers, achieving consistent and reproducible solid-phase extraction (SPE) sample preparation is one of the biggest goals every day. The Otto SPEcialist Positive Pressure Manifold is made to address the common challenges of reproducibility, method transfer, and processing efficiency in SPE sample preparation. Designed to reduce user variability, this semi-automated system offers high-quality sample preparation and extraction through its innovative programmed pressure profiles and methods.
At the core of the Otto SPEcialist Positive Pressure Manifold is the Otto software, an interfaced tablet control system that simplifies SPE method development, positive pressure optimization, and real-time manipulation of pressure profiles. This software is designed for both novice and expert users, requiring little to no training. The intuitive touchscreen interface allows users to easily create and adjust pressure profiles to optimize flow rates for each step of their SPE processes. Whether dealing with simple or complex workflows, the Otto software ensures method robustness and consistency.
The Otto SPEcialist Positive Pressure Manifold is compatible with a wide range of SPE plates and cartridges, including µElution and macro elution plates, as well as 1 cc, 3 cc, and 6 cc cartridges. This versatility allows for the processing of various sample volumes, accommodating the diverse needs of SPE applications. By eliminating technique variation due to differing levels of user expertise, the Otto SPEcialist ensures consistent performance across SPE runs, making it an invaluable tool for laboratories focused on analytical consistency and reproducibility.
One of the key benefits of the Otto SPEcialist is its ability to perform multiple workflows in a single day. The system's flexibility in handling very low to high flow rates for sorbent bed drying ensures that a wide range of SPE processing needs are met. The compact design of the device allows it to fit easily on a bench or under a hood, making it a convenient addition to any laboratory setting.
This Manifold excels in ensuring consistency across users and labs. By running programmed methods that include a combination of pressure profiles and manifold commands, the system eliminates user-to-user variability. The Otto software meticulously tracks and documents each pressure profile and method processed, ensuring that workflow consistency is maintained. This feature is particularly beneficial for laboratories where multiple users operate the system, as it guarantees that the same high-quality results are achieved regardless of who is running the samples.
The Otto SPEcialist Positive Pressure Manifold is a cutting-edge solution for improving the reproducibility and efficiency of SPE sample preparation. Its easy-to-use software, compatibility with various SPE plates and cartridges, and ability to perform multiple workflows in a single day make it a versatile and reliable tool for any laboratory. By eliminating user variability and ensuring consistent performance, the Otto SPEcialist sets a new standard in solid-phase extraction technology, making high-quality sample preparation accessible to all.
