RapiFluor-MS Glycan Performance Test Standard

As part of Waters’ complete, workflow-based approach to analyzing glycoproteins at all structural levels, the RapiFluor-MS Glycan Performance Test Standard is intended to be used as a benchmarking standard. This labeled, complex control complements the GlycoWorks RapiFluor-MS N-Glycan Kit solution. Each vial of the test standard contains the N-Glycan content from 30 µg of pooled human IgG (Sigma I4506), or roughly 400 pmols of RapiFluor-MS labeled, released N-glycans.

The RapiFluor-MS reagent is part of Waters’ reimagined workflow for released N-glycan analysis and features the ionization capability necessary to maximize the information generated by MS detection. This workflow speeds up deglycosylation and streamlines labeling. With this innovation, laboratories no longer have to choose between a “quick” labeling tool and sensitivity for mass spectrometry and have no more labor-intensive, complicated, and error-prone processes. With the use of Waters’ chemistries and lab equipment, you can be confident in your analytical results without compromising between speed and sensitivity.

As part of the Glycan Monitoring system and in conjunction with the ACQUITY QDa Detector, mass detection of glycans with unrivaled sensitivity for FLR and MS detection is possible with greater confidence with mass confirmation of FLR peaks. This also allows for reduction of chromatography analysis times, with targeted MS glycan profiling for rapid process development. Easy integration is possible with existing GMP-compliant ready UPLC workflows using Empower 3 software.

For the best results, recommended experimental conditions include the use of the ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 150K, 1/pk. This complimentary lab equipment is sure to take your work to the next level of expertise and efficiency to ensure optimal results every time. A stainless steel needle is recommended when injecting samples contain DMF as a co-solvent.