Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
RapiGest SF, is a patented anionic surfactant that drastically improves in-solution protein digestion in terms of speed and peptide recovery. It is especially valuable in protein sample preparation for mass spectrometry (MS) analysis by avoiding high background noise often seen from other common surfactants.
RapiGest SF is available in a variety of formats. This part contains one, 3 mg vial. Add RapiGest SF to your proteolytic digestion, (using trypsin, PNGase F or other enzymes) to enhance digestion speed and increase peptide recovery.
Improve protein enzymatic digestions in terms of speed and percent recovery with the Waters RapiGest SF(surfactant). RapiGest is a patented anionic surfactant that speeds up the in-solution production of peptides generated by proteases, such as trypsin, Asp-N, Glu-C, and Lys-C. Solubilize and unfold proteins with the RapiGest SF by making them more amenable to cleavage without denaturing or inhibiting common proteolytic enzymes as it is a mild denaturant. This is significant since many hydrophobic proteins are resistant to proteolysis because their cleavage sites are inaccessible to endoproteases.
Some of the benefits of using the RapiGest SF include improving the protein sequence coverage and reducing sample preparation time. It also reduces digestion time and requires fewer enzymes to achieve optimum digestion. Digestion of enzyme-resistant proteins such as membrane proteins can also be improved with RapiGest SF. It is especially valuable in protein sample preparation for mass spectrometry (MS) analysis, as it avoids high background noise often seen from other common surfactants, making it a valuable addition to your lab equipment.
This particular listing contains one vial of 3mg of RapiGest SF, and you can use it to enhance digestion speed and increase peptide recovery. The RapiGest SF is available in other formats, and the vial sizes vary from 1 mg to 10 mg and 50 mg. You can browse through our brochure or review our website to see the additional offerings and other equipment offered by Waters to shop for lab equipment as per your laboratory needs.
You might also be interested in reviewing the LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk, all Waters LCMS Certified vials are produced following tightly controlled manufacturing processes and handling procedures to ensure they are inert and free from any contaminants and impurities. The vials are tested with mass spectrometers and come packaged with a certificate of analysis showing the reference and vial scan for the manufacturing lot.
What Can Be Done To Enhance Enrichment In Sample Components Present In Low Concentration?
Several steps can be taken to enrich sample components present in low concentrations. You can tailor the gradient steps to elute analytes selectively. Another effective method is to use "large" sample volumes in an adsorption-promoting solvent. In a desorption-promoting solvent, use a "small" collection volume. Use sorbent chemistry tailored to the analyte, independent of that in the analytical column. The chemistry of the solid-phase extraction column should be carefully chosen, so no further sample prep is required.
