mAb Tryptic Digestion Standard

Peptide level analysis of proteins by LC-MS is one of the primary approaches for the characterization of biopharmaceuticals, in particular monoclonal antibodies (mAbs). In such an analysis, it is possible to acquire peptide mass information, quickly confirm protein sequence, and utilize post-translational modifications, as well as degradation-related critical quality attributes.

To support this application area, Waters has commercialized the mAb Tryptic Digestion Standard, which can be used in the benchmarking of LC-MS techniques, proficiency testing among different lab equipment and laboratories, and system suitability when performing peptide level protein analysis. This standard is a stabilized and lyophilized format of a reduced and alkylated tryptic digest of NIST Reference Material 8671 (NIST mAb), a humanized IgG1κ expressed from a murine cell line. Each vial of the standard is provided in an approximately 40 µg quantity.

Standards and reagents are critical components for assuring maximum performance and compliance with analytical instruments, and Waters offers a selection of standard kits to fit your individual needs. It is vital to have lab equipment that can keep up as the technical and regulatory requirements for developing, documenting, and testing analytical standards and reagents become more complex and time-consuming. Opt for Waters Analytical Standards and Reagents in your lab as they help you increase your efficiency and compliance. Use our online store to purchase lab equipment directly from us.

These products may be categorized as hazardous after use, depending on the user's use, and are intended to be handled by professional laboratory staff trained in the safe handling of such chemicals.

You may also be interested in the BioResolve RP mAb Polyphenyl Column, 450Å, 2.7 µm, 3 mm X 100 mm, 1/pk with Intact mAb and mAb Subunit Standard?. The lab equipment contains 2.7-μm superficially-porous-silica particles having an average pore diameter of 450Å. The particles are functionalized with a proprietary polyphenyl bonding process that was specifically designed for LC or LC-MS analyses of intact IgG monoclonal antibodies and their fragments.